PMID- 15955303
OWN - NLM
STAT- MEDLINE
DCOM- 20050728
LR  - 20231213
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1711
IP  - 2
DP  - 2005 Jun 10
TI  - Sensitivity of the brain transcriptome to connexin ablation.
PG  - 183-96
AB  - Extensive studies on mice with total or partial disruption of either connexin43 
      (Cx43) or connexin32 (Cx32) have detected only subtle changes in central nervous 
      system structure, growth, development, or function. We have used high density 
      cDNA arrays to analyze the regulation, control, and coordination of the 
      abundances of 7446 distinct transcripts in four brains, each of Cx43 null (K43), 
      Cx43 heterozygous (H43), and Cx32 null (K32) mice as compared to the brains of 
      wildtype (W) mice. The use of multiple samples allowed the determination of the 
      statistical significance of gene regulation. Significantly regulated genes 
      encoded proteins of all functional categories, extending beyond those that might 
      be expected to depend on junctional communication. Moreover, we found a high 
      degree of similarity between genes regulated in the K43 and H43 brains and a 
      remarkable overlap between gene regulation in brains of K43 and K32. The 
      regulated genes in both K43 and H43 brains showed an outstanding inverse 
      coordination with the levels of expression of Cx43 in W brain, indicating that 
      the regulated genes are largely predictable from their co-variance with Cx43 in 
      the wildtype samples. These findings lead to the hypothesis that connexin 
      expression may represent a central node in the regulation of gene expression 
      patterns in brain.
FAU - Iacobas, Dumitru A
AU  - Iacobas DA
AD  - Department of Neuroscience, Albert Einstein College of Medicine, 1410 Pelham 
      Parkway South, Kennedy Center, Room 915C, New York, NY 10461, United States. 
      diacobas@aecom.yu.edu
FAU - Iacobas, Sanda
AU  - Iacobas S
FAU - Urban-Maldonado, Marcia
AU  - Urban-Maldonado M
FAU - Spray, David C
AU  - Spray DC
LA  - eng
GR  - DK41918/DK/NIDDK NIH HHS/United States
GR  - MH65495/MH/NIMH NIH HHS/United States
GR  - NS41282/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
DEP - 20041222
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Connexin 43)
RN  - 0 (Connexins)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Animals
MH  - Brain/metabolism
MH  - Connexin 43/deficiency/*physiology
MH  - Connexins/deficiency/*physiology
MH  - Gene Expression Regulation
MH  - Mice
MH  - Mice, Knockout
MH  - Nerve Tissue Proteins/*biosynthesis
MH  - Oligonucleotide Array Sequence Analysis/methods
MH  - Transcription, Genetic
MH  - Gap Junction beta-1 Protein
RF  - 67
EDAT- 2005/06/16 09:00
MHDA- 2005/07/29 09:00
CRDT- 2005/06/16 09:00
PHST- 2004/07/29 00:00 [received]
PHST- 2004/11/24 00:00 [revised]
PHST- 2004/12/02 00:00 [accepted]
PHST- 2005/06/16 09:00 [pubmed]
PHST- 2005/07/29 09:00 [medline]
PHST- 2005/06/16 09:00 [entrez]
AID - S0005-2736(04)00317-7 [pii]
AID - 10.1016/j.bbamem.2004.12.002 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2005 Jun 10;1711(2):183-96. doi: 
      10.1016/j.bbamem.2004.12.002. Epub 2004 Dec 22.