PMID- 15956966
OWN - NLM
STAT- MEDLINE
DCOM- 20050912
LR  - 20220216
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 92
IP  - 12
DP  - 2005 Jun 20
TI  - Decreased expression of haemoglobin beta (HBB) gene in anaplastic thyroid cancer 
      and recovery of its expression inhibits cell growth.
PG  - 2216-24
AB  - Anaplastic thyroid cancer (ATC) is one of the most fulminant and foetal diseases 
      in human malignancies. However, the genetic alterations and carcinogenic 
      mechanisms of ATC are still unclear. Recently, we investigated the gene 
      expression profile of 11 anaplastic thyroid cancer cell lines (ACL) and 
      significant decreased expression of haemoglobin beta (HBB) gene in ACL. 
      Haemoglobin beta is located at 11p15.5, where loss of heterozygosity (LOH) was 
      reported in various kinds of cancers, including ATC, and it has been suggested 
      that novel tumour suppressor genes might exist in this region. In order to 
      clarify the meaning of decreased expression of HBB in ATC, the expression status 
      of HBB was investigated with ACL, ATC, papillary thyroid cancer (PTC) and normal 
      human tissues. Haemoglobin beta showed significant decreased expression in ACLs 
      and ATCs; however, in PTC, HBB expressed equal to the normal thyroid gland. In 
      addition, HBB expressed in normal human tissues ubiquitously. To validate the 
      tumour-suppressor function of HBB, cell growth assay was performed. Forced 
      expression of HBB in KTA2 cell, which is a kind of ACL, significantly suppressed 
      KTA2 growth. The mechanism of downregulation of HBB in ATC is still unclear; 
      however, our results suggested the possibility of HBB as a novel 
      tumour-suppressor gene.
FAU - Onda, M
AU  - Onda M
AD  - Department of Molecular Biology, Institute of Gerontology, Nippon Medical School, 
      Kawasaki, Japan. monda@nms.ac.jp
FAU - Akaishi, J
AU  - Akaishi J
FAU - Asaka, S
AU  - Asaka S
FAU - Okamoto, J
AU  - Okamoto J
FAU - Miyamoto, S
AU  - Miyamoto S
FAU - Mizutani, K
AU  - Mizutani K
FAU - Yoshida, A
AU  - Yoshida A
FAU - Ito, K
AU  - Ito K
FAU - Emi, M
AU  - Emi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - 0 (Hemoglobins)
RN  - 9004-22-2 (Globins)
SB  - IM
MH  - Carcinoma/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Gene Expression Profiling
MH  - Genes, Tumor Suppressor
MH  - Globins/*genetics
MH  - Hemoglobins/genetics
MH  - Humans
MH  - Thyroid Neoplasms/*genetics/pathology
PMC - PMC2361827
EDAT- 2005/06/16 09:00
MHDA- 2005/09/13 09:00
PMCR- 2006/06/20
CRDT- 2005/06/16 09:00
PHST- 2005/06/16 09:00 [pubmed]
PHST- 2005/09/13 09:00 [medline]
PHST- 2005/06/16 09:00 [entrez]
PHST- 2006/06/20 00:00 [pmc-release]
AID - 6602634 [pii]
AID - 10.1038/sj.bjc.6602634 [doi]
PST - ppublish
SO  - Br J Cancer. 2005 Jun 20;92(12):2216-24. doi: 10.1038/sj.bjc.6602634.