PMID- 15958709
OWN - NLM
STAT- MEDLINE
DCOM- 20061113
LR  - 20071114
IS  - 0077-8923 (Print)
IS  - 0077-8923 (Linking)
VI  - 1044
DP  - 2005 Jun
TI  - Gene expression in stem cell-supporting stromal cell lines.
PG  - 159-67
AB  - Cells in the immediate microenvironment together with hematopoietic stem cells 
      (HSCs) constitute the stem cell niche. The microenvironmental or stromal cells 
      provide a complex molecular milieu that helps mediate and balance the 
      self-renewal and commitment potentials of stem cells. The molecules in this 
      milieu are not well defined. In this study, we have intersected previous cDNA 
      subtraction studies with array expression methodologies to define and categorize 
      known gene products expressed by HSC-supportive stromal cell lines. Data were 
      curated from our previously released Stromal Cell Database (StroCDB) containing a 
      set of gene products enriched for expression in the fetal liver stromal cell line 
      AFT024. Global expression analyses were extended to other stem cell-supporting 
      and -nonsupporting fetal liver stromal cell lines using commercially available 
      microarrays. Known and previously described gene products from selected 
      categories were studied: transcription factors, cell membrane proteins, 
      cytoskeleton and related proteins, extracellular matrix proteins, cell adhesion 
      molecules and their corresponding signaling molecules, and cytokines and their 
      related mediators. More than 300 known gene products were selected for expression 
      in HSC-supporting stromal cells compared to nonsupporting lines. Analyses of the 
      data suggest that HSC-supportive cells are immature, sessile, and highly reactive 
      after binding to integrin ligands and cytokines. Therefore, they provide a 
      dynamic space poised to respond to molecular cues elaborated within the stem cell 
      niche. The study provides a survey of known proteins that play key roles in the 
      support of HSCs by fetal liver stromal cells. It also provides insights into the 
      biology of the stem cell niche by highlighting the complex network of 
      intercellular signaling and communication involved in the organization of the 
      niche space.
FAU - Charbord, Pierre
AU  - Charbord P
AD  - Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
FAU - Moore, Kateri
AU  - Moore K
LA  - eng
GR  - HL58739/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - *Gene Expression
MH  - Gene Expression Profiling
MH  - *Hematopoietic Stem Cells/cytology/physiology
MH  - Humans
MH  - Liver/cytology/embryology
MH  - Models, Biological
MH  - Stromal Cells/cytology/*physiology
RF  - 27
EDAT- 2005/06/17 09:00
MHDA- 2006/11/14 09:00
CRDT- 2005/06/17 09:00
PHST- 2005/06/17 09:00 [pubmed]
PHST- 2006/11/14 09:00 [medline]
PHST- 2005/06/17 09:00 [entrez]
AID - 1044/1/159 [pii]
AID - 10.1196/annals.1349.020 [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2005 Jun;1044:159-67. doi: 10.1196/annals.1349.020.