PMID- 15958750
OWN - NLM
STAT- MEDLINE
DCOM- 20060214
LR  - 20211203
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 25
IP  - 24
DP  - 2005 Jun 15
TI  - Local protein synthesis mediates a rapid increase in dendritic elongation factor 
      1A after induction of late long-term potentiation.
PG  - 5833-43
AB  - The maintenance of long-term potentiation (LTP) requires a brief period of 
      accelerated protein synthesis soon after synaptic stimulation, suggesting that an 
      early phase of enhanced translation contributes to stable LTP. The mechanism 
      regulating protein synthesis and the location and identities of mRNAs translated 
      are not well understood. Here, we show in acute brain slices that the induction 
      of protein synthesis-dependent hippocampal LTP increases the expression of 
      elongation factor 1A (eEF1A), the mRNA of which contains a 5' terminal 
      oligopyrimidine tract. This effect is blocked by rapamycin, indicating that the 
      increase in EF1A expression is mediated by the mammalian target of rapamycin 
      (mTOR) pathway. We find that mRNA for eEF1A is present in pyramidal cell 
      dendrites and that the LTP-associated increase in eEF1A expression was intact in 
      dendrites that had been severed from their cell bodies before stimulation. eEF1A 
      levels increased within 5 min after stimulation in a translation-dependent 
      manner, and this effect remained stable for 3 h. These results suggest a 
      mechanism whereby synaptic stimulation, by signaling through the mTOR pathway, 
      produces an increase in dendritic translational capacity that contributes to LTP 
      maintenance.
FAU - Tsokas, Panayiotis
AU  - Tsokas P
AD  - Department of Pharmacology and Biological Chemistry, Mount Sinai School of 
      Medicine, New York, New York 10029, USA.
FAU - Grace, Elizabeth A
AU  - Grace EA
FAU - Chan, Pokman
AU  - Chan P
FAU - Ma, Tao
AU  - Ma T
FAU - Sealfon, Stuart C
AU  - Sealfon SC
FAU - Iyengar, Ravi
AU  - Iyengar R
FAU - Landau, Emmanuel M
AU  - Landau EM
FAU - Blitzer, Robert D
AU  - Blitzer RD
LA  - eng
GR  - R56 DK046943/DK/NIDDK NIH HHS/United States
GR  - DA017135/DA/NIDA NIH HHS/United States
GR  - GM54508/GM/NIGMS NIH HHS/United States
GR  - R01 DK046943/DK/NIDDK NIH HHS/United States
GR  - DA15863/DA/NIDA NIH HHS/United States
GR  - DK46943/DK/NIDDK NIH HHS/United States
GR  - R01 DA015863/DA/NIDA NIH HHS/United States
GR  - R01 GM054508/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Peptide Elongation Factor 1)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Western
MH  - Dendrites/*physiology
MH  - Gene Expression Regulation
MH  - Immunohistochemistry
MH  - Long-Term Potentiation/*physiology
MH  - Male
MH  - Molecular Sequence Data
MH  - Peptide Elongation Factor 1/biosynthesis/*genetics
MH  - *Protein Biosynthesis
MH  - Protein Kinases/genetics
MH  - RNA, Messenger/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases
PMC - PMC6724870
EDAT- 2005/06/17 09:00
MHDA- 2006/02/16 09:00
PMCR- 2005/12/15
CRDT- 2005/06/17 09:00
PHST- 2005/06/17 09:00 [pubmed]
PHST- 2006/02/16 09:00 [medline]
PHST- 2005/06/17 09:00 [entrez]
PHST- 2005/12/15 00:00 [pmc-release]
AID - 25/24/5833 [pii]
AID - 10.1523/JNEUROSCI.0599-05.2005 [doi]
PST - ppublish
SO  - J Neurosci. 2005 Jun 15;25(24):5833-43. doi: 10.1523/JNEUROSCI.0599-05.2005.