PMID- 15961574
OWN - NLM
STAT- MEDLINE
DCOM- 20060111
LR  - 20240109
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 78
IP  - 3
DP  - 2005 Sep
TI  - Polarization of naive T cells into Th1 or Th2 by distinct cytokine-driven murine 
      dendritic cell populations: implications for immunotherapy.
PG  - 656-64
AB  - Dendritic cells (DCs) activate T cells and regulate their differentiation into T 
      helper cell type 1 (Th1) and/or Th2 cells. To identify DCs with differing 
      abilities to direct Th1/Th2 cell differentiation, we cultured mouse bone marrow 
      progenitors in granulocyte macrophage-colony stimulating factor (GM), GM + 
      interleukin (IL)-4, or GM + IL-15 and generated three distinct DC populations. 
      The GM + IL-4 DCs expressed high levels of CD80/CD86 and major histocompatibility 
      complex (MHC) class II and produced low levels of IL-12p70. GM and GM + IL-15 DCs 
      expressed low levels of CD80/CD86 and MHC class II. The GM + IL-15 DCs produced 
      high levels of IL-12p70 and interferon (IFN)-gamma, whereas GM DCs produced only 
      high levels of IL-12p70. Naive T cells stimulated with GM + IL-4 DCs secreted 
      high levels of IL-4 and IL-5 in addition to IFN-gamma. In contrast, the GM + 
      IL-15 DCs induced higher IFN-gamma production by T cells with little or no Th2 
      cytokines. GM DCs did not induce T cell polarization, despite producing large 
      amounts of IL-12p70 following activation. A similar pattern of T cell activation 
      was observed after in vivo administration of DCs. These data suggest that 
      IL-12p70 production alone, although necessary for Th1 differentiation, is not 
      sufficient to induce Th1 responses. These studies have implications for the use 
      of DC-based vaccines in immunotherapy of cancer and other clinical conditions.
FAU - Feili-Hariri, Maryam
AU  - Feili-Hariri M
AD  - Division of Plastic Surgery, University of Pittsburgh, Scaife Hall, Suite 666, 
      3550 Terrace St., Pittsburgh, PA 15261, USA. haririmf@upmc.edu
FAU - Falkner, Dewayne H
AU  - Falkner DH
FAU - Morel, Penelope A
AU  - Morel PA
LA  - eng
GR  - CA73743/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050616
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Interleukin-15)
RN  - 0 (Protein Subunits)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - B7-1 Antigen/biosynthesis/drug effects
MH  - B7-2 Antigen/biosynthesis/drug effects
MH  - CD4-Positive T-Lymphocytes/drug effects/*immunology/metabolism
MH  - Cell Differentiation/drug effects/immunology
MH  - Cell Polarity/drug effects/immunology/physiology
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/drug effects/*immunology
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - *Immunotherapy
MH  - Interferon-gamma/biosynthesis/drug effects
MH  - Interleukin-12/biosynthesis
MH  - Interleukin-15/biosynthesis/immunology/pharmacology
MH  - Interleukin-4/biosynthesis/immunology/pharmacology
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, Inbred Strains
MH  - Mice, Transgenic
MH  - Phenotype
MH  - Protein Subunits/biosynthesis/drug effects
MH  - Th1 Cells/drug effects/*immunology
MH  - Th2 Cells/drug effects/*immunology
EDAT- 2005/06/18 09:00
MHDA- 2006/01/13 09:00
CRDT- 2005/06/18 09:00
PHST- 2005/06/18 09:00 [pubmed]
PHST- 2006/01/13 09:00 [medline]
PHST- 2005/06/18 09:00 [entrez]
AID - jlb.1104631 [pii]
AID - 10.1189/jlb.1104631 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2005 Sep;78(3):656-64. doi: 10.1189/jlb.1104631. Epub 2005 Jun 16.