PMID- 15961724
OWN - NLM
STAT- MEDLINE
DCOM- 20051025
LR  - 20161124
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 33
IP  - 4
DP  - 2005 Oct
TI  - Diacylglycerol-containing docosahexaenoic acid in acyl chain modulates airway 
      smooth muscle tone.
PG  - 378-86
AB  - We synthesized and assessed the role of a diacylglycerol (DAG)-containing 
      docosahexaenoic acid (DHA), that is, 1-stearoyl-2-docosahexaenoyl-sn-glycerol 
      (SDHG), in the contraction of guinea pig airway smooth muscle (ASM). We compared 
      its action with 1-stearoyl-2-arachidonoyl-sn-glycerol (SAG) and 
      1,2-dioctanoyl-sn-glycerol (1,2-DiC8), a stable DAG analog. The three DAGs (SAG, 
      SDHG, and 1,2-DiC8) induced reversible concentration-dependent contraction of 
      ASM. SDHG induced higher guinea pig ASM contraction than did SAG and 1,2-DiC8. 
      The effects of SDHG were blocked, to different extents, by nifedipine (L-type 
      Ca2+ channel blocker). By employing GF-109203X (protein kinase C [PKC] inhibitor) 
      and lanthanum (La3+), a nonselective cation channel blocker, we observed that 
      SDHG evoked ASM contractile response via PKC-dependent and PKC-independent (but 
      Ca2+-dependent) pathways. Interestingly, SAG exerted its action only by 
      increasing [Ca2+]i and did not require PKC activation. To probe the implication 
      of calcium mobilization, we employed thapsigargin (TG), which also induced ASM 
      contraction in a calcium-dependent manner. SDHG and 1,2-DiC8, in a PKC-dependent 
      manner, induced the phosphorylation of CPI-17 (myosin light chain phosphatase 
      inhibitor of 17 kD). Furthermore, SAG and TG failed to phosphorylate CPI-17 in 
      ASM cells. Our results suggest that different DAG species, produced during a 
      dietary supplementation with fatty acids, could modulate the reactivity of airway 
      smooth muscles in a PKC-dependent and -independent manner, and hence, may play a 
      critical role in health and disease.
FAU - Hichami, Aziz
AU  - Hichami A
AD  - Departement-Physiologie, Immunologie et Neurosciences, UPRES Lipides et 
      Nutrition, Faculte des Sciences de la Vie, 21000 Dijon, France.
FAU - Morin, Caroline
AU  - Morin C
FAU - Rousseau, Eric
AU  - Rousseau E
FAU - Khan, Naim A
AU  - Khan NA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050616
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Diglycerides)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indoles)
RN  - 0 (Maleimides)
RN  - 25167-62-8 (Docosahexaenoic Acids)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - I9ZF7L6G2L (Nifedipine)
RN  - L79H6N0V6C (bisindolylmaleimide I)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Bronchi/*anatomy & histology
MH  - Calcium/metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Cells, Cultured
MH  - Diglycerides/chemistry/*metabolism
MH  - *Docosahexaenoic Acids/chemistry/metabolism
MH  - Enzyme Activation
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Guinea Pigs
MH  - Humans
MH  - In Vitro Techniques
MH  - Indoles/pharmacology
MH  - Male
MH  - Maleimides/pharmacology
MH  - Muscle Contraction/drug effects/physiology
MH  - Muscle, Smooth/drug effects/*physiology
MH  - Nifedipine/pharmacology
MH  - Phosphorylation
MH  - Protein Kinase C/antagonists & inhibitors/metabolism
EDAT- 2005/06/18 09:00
MHDA- 2005/10/26 09:00
CRDT- 2005/06/18 09:00
PHST- 2005/06/18 09:00 [pubmed]
PHST- 2005/10/26 09:00 [medline]
PHST- 2005/06/18 09:00 [entrez]
AID - 2005-0136OC [pii]
AID - 10.1165/rcmb.2005-0136OC [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 2005 Oct;33(4):378-86. doi: 10.1165/rcmb.2005-0136OC. 
      Epub 2005 Jun 16.