PMID- 15963827
OWN - NLM
STAT- MEDLINE
DCOM- 20051026
LR  - 20061115
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 106
IP  - 3-4
DP  - 2005 Jul 15
TI  - Protective immunity induced by a recombinant pseudorabies virus expressing the 
      GP5 of porcine reproductive and respiratory syndrome virus in piglets.
PG  - 309-19
AB  - Pseudorabies virus (PRV) has been developed as a vaccine vector for expressing 
      foreign immunogens. Porcine reproductive and respiratory syndrome (PRRS), caused 
      by porcine reproductive and respiratory syndrome virus (PRRSV), continues to be a 
      major problem to the pork industry worldwide. Many vaccine strategies have been 
      developed to control the disease but most of them turn out to be unsuccessful. 
      The objective of this research was to explore the feasibility of PRV-based vector 
      vaccine in protection against PRRSV. A live attenuated vaccine-based PRV 
      recombinant expressing the envelope protein GP5 of PRRSV was generated using 
      recombinant DNA techniques. The Bartha-K61-derived recombinant virus, named 
      rPRV-GP5, was shown to express PRRSV GP5 efficiently. Sixteen healthy piglets 
      were assigned to one of four groups (one to four, four pigs per group). Animals 
      in Groups 1 and 2 were each inoculated intramuscularly and intranasally with 
      10(7.0) PFU of rPRV-GP5 and its parent Bartha-K61, respectively; Group 3 were 
      vaccinated intramuscularly with one-dose of PRRS inactivated vaccine; Group 4 was 
      served as non-vaccinated control. One month later, all animals were all 
      challenged with 10(6.5) TCID(50) of virulent PRRSV CH-1a. All animals in Groups 1 
      and 3 remained clinically healthy before and after challenge, with only a short 
      period of fever (no more than 41 degrees C and 3 days), mild and gradually 
      improving lung and kidney lesions, and short-term viremia (2 and 3 week, 
      respectively) in spite of no detectable anti-PRRSV antibody before challenge. On 
      the other hand, all animals in the other two groups showed evident clinical signs 
      with higher temperatures (more than 41 degrees C) after challenge, and severe 
      lung, kidney and spleen lesions and extended viremia (4 weeks). The results 
      indicate that the rPRV-GP5 is safe for vaccinates and able to confer significant 
      protection against clinical disease and reduce pathogenic lesions induced by 
      PRRSV challenge in vaccinated pigs.
FAU - Qiu, Hua-Ji
AU  - Qiu HJ
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, 
      Harbin 150001, PR China.
FAU - Tian, Zhi-Jun
AU  - Tian ZJ
FAU - Tong, Guang-Zhi
AU  - Tong GZ
FAU - Zhou, Yan-Jun
AU  - Zhou YJ
FAU - Ni, Jian-Qiang
AU  - Ni JQ
FAU - Luo, Yu-Zi
AU  - Luo YZ
FAU - Cai, Xue-Hui
AU  - Cai XH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Administration, Intranasal
MH  - Animals
MH  - Genetic Vectors
MH  - Herpesvirus 1, Suid/*genetics/*immunology
MH  - Injections, Intramuscular
MH  - Organ Specificity
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/pathology/*prevention & 
      control/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/*immunology/isolation & purification/pathogenicity
MH  - Sus scrofa
MH  - Vaccines, Synthetic/administration & dosage/genetics/immunology
MH  - Viral Envelope Proteins/*genetics/*immunology
MH  - Viral Vaccines/administration & dosage/genetics/immunology
MH  - Viremia/immunology/prevention & control
EDAT- 2005/06/21 09:00
MHDA- 2005/10/27 09:00
CRDT- 2005/06/21 09:00
PHST- 2004/11/17 00:00 [received]
PHST- 2005/03/01 00:00 [revised]
PHST- 2005/03/21 00:00 [accepted]
PHST- 2005/06/21 09:00 [pubmed]
PHST- 2005/10/27 09:00 [medline]
PHST- 2005/06/21 09:00 [entrez]
AID - S0165-2427(05)00113-3 [pii]
AID - 10.1016/j.vetimm.2005.03.008 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2005 Jul 15;106(3-4):309-19. doi: 
      10.1016/j.vetimm.2005.03.008.