PMID- 15964511
OWN - NLM
STAT- MEDLINE
DCOM- 20051024
LR  - 20131121
IS  - 0891-5849 (Print)
IS  - 0891-5849 (Linking)
VI  - 39
IP  - 2
DP  - 2005 Jul 15
TI  - Long-term melatonin or 17beta-estradiol supplementation alleviates oxidative 
      stress in ovariectomized adult rats.
PG  - 195-204
AB  - Melatonin is an endogenously generated potent antioxidant. Our previous results 
      indicated that melatonin improved learning and memory deficits in the transgenic 
      mouse model of Alzheimer's disease (AD) and ovariectomized (OVX) rats by 
      improving cholinergic nerve system dysfunction, preventing apoptosis. In this 
      study we aim to investigate the antioxidative effects of melatonin or estradiol 
      in the brains of ovariectomized rats. OVX Sprague-Dawley rats received daily 
      injections of melatonin (5, 10, or 20 mg/kg), 17beta-estradiol (80 microg/kg), or 
      sesame oil for 16 weeks. We found an increase in brain mitochondrial 
      thiobarbituric acid-reactive substances (TBARS) levels, a decrease in 
      mitochondrial glutathione (GSH) content as well as mitochondrial superoxide 
      dismutase (SOD) activity and upregulation of the apoptotic-related factors, such 
      as Bax, Caspase-3, and Prostate apoptosis response-4 (Par-4) in the frontal 
      cortex of OVX rats. In addition to oxidative stress, OVX also caused decreased 
      activities of mitochondrial respiration complex I and complex IV, which 
      implicated mitochondrial dysfunction. Melatonin or 17beta-estradiol antagonized 
      the detrimental effects induced by OVX. Furthermore, immunohistochemistry results 
      revealed that the abnormal upregulation of the apoptotic related factor such as 
      Bax, Caspase-3, and (Par-4) greatly reduced expression after melatonin or 
      17beta-estradiol supplement action. These findings demonstrate the important 
      effects of melatonin or 17beta-estradiol on postmenopausal neuropathy and support 
      the potential application of melatonin in the treatment of dementia in 
      postmenopausal women. Early, long-term melatonin application is a promising 
      strategy which could potentially be applied in a clinical setting.
FAU - Feng, Zheng
AU  - Feng Z
AD  - Department of Pharmacology, Institute of Materia Medica, Chinese Academy of 
      Medical Sciences, Peking Union Medical College, No.1 Xiannong Tan Street, Beijing 
      10050, People's Republic of China.
FAU - Zhang, Jun-tian
AU  - Zhang JT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050325
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - 0 (Antioxidants)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 0 (prostate apoptosis response-4 protein)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.4.22.- (Casp3 protein, mouse)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
RN  - GAN16C9B8O (Glutathione)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Apoptosis Regulatory Proteins
MH  - Brain/metabolism
MH  - Caspase 3
MH  - Caspases/metabolism
MH  - Dietary Supplements
MH  - Electron Transport
MH  - Estradiol/*pharmacology
MH  - Female
MH  - Glutathione/metabolism
MH  - Immunohistochemistry
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Lipid Peroxidation
MH  - Melatonin/metabolism/*pharmacology
MH  - Mitochondria/metabolism
MH  - Ovary/pathology
MH  - Oxidative Stress/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Superoxide Dismutase/metabolism
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - Time Factors
MH  - Up-Regulation
EDAT- 2005/06/21 09:00
MHDA- 2005/10/26 09:00
CRDT- 2005/06/21 09:00
PHST- 2004/12/14 00:00 [received]
PHST- 2005/02/25 00:00 [revised]
PHST- 2005/03/07 00:00 [accepted]
PHST- 2005/06/21 09:00 [pubmed]
PHST- 2005/10/26 09:00 [medline]
PHST- 2005/06/21 09:00 [entrez]
AID - S0891-5849(05)00133-4 [pii]
AID - 10.1016/j.freeradbiomed.2005.03.007 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 2005 Jul 15;39(2):195-204. doi: 
      10.1016/j.freeradbiomed.2005.03.007. Epub 2005 Mar 25.