PMID- 15964824
OWN - NLM
STAT- MEDLINE
DCOM- 20050802
LR  - 20181113
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 25
IP  - 13
DP  - 2005 Jul
TI  - Transcription factor KLF7 is important for neuronal morphogenesis in selected 
      regions of the nervous system.
PG  - 5699-711
AB  - The Kruppel-like transcription factors (KLFs) are important regulators of cell 
      proliferation and differentiation in several different organ systems. The mouse 
      Klf7 gene is strongly active in postmitotic neuroblasts of the developing nervous 
      system, and the corresponding protein stimulates transcription of the 
      cyclin-dependent kinase inhibitor p21waf/cip gene. Here we report that loss of 
      KLF7 activity in mice leads to neonatal lethality and a complex phenotype which 
      is associated with deficits in neurite outgrowth and axonal misprojection at 
      selected anatomical locations of the nervous system. Affected axon pathways 
      include those of the olfactory and visual systems, the cerebral cortex, and the 
      hippocampus. In situ hybridizations and immunoblots correlated loss of KLF7 
      activity in the olfactory epithelium with significant downregulation of the 
      p21waf/cip and p27kip1 genes. Cotransfection experiments extended the last 
      finding by documenting KLF7's ability to transactivate a reporter gene construct 
      driven by the proximal promoter of p27kip1. Consistent with emerging evidence for 
      a role of Cip/Kip proteins in cytoskeletal dynamics, we also documented 
      p21waf/cip and p27kip1 accumulation in the cytoplasm of differentiating olfactory 
      sensory neurons. KLF7 activity might therefore control neuronal morphogenesis in 
      part by optimizing the levels of molecules that promote axon outgrowth.
FAU - Laub, Friedrich
AU  - Laub F
AD  - Laboratory of Genetics and Organogenesis, Research Division of the Hospital for 
      Special Surgery, and Department of Physiology and Biophysics at Weill Medical 
      College of Cornell University, 535 East 70th St., New York, New York 10021, USA.
FAU - Lei, Lei
AU  - Lei L
FAU - Sumiyoshi, Hideaki
AU  - Sumiyoshi H
FAU - Kajimura, Daisuke
AU  - Kajimura D
FAU - Dragomir, Cecilia
AU  - Dragomir C
FAU - Smaldone, Silvia
AU  - Smaldone S
FAU - Puche, Adam C
AU  - Puche AC
FAU - Petros, Timothy J
AU  - Petros TJ
FAU - Mason, Carol
AU  - Mason C
FAU - Parada, Luis F
AU  - Parada LF
FAU - Ramirez, Francesco
AU  - Ramirez F
LA  - eng
GR  - AR38648/AR/NIAMS NIH HHS/United States
GR  - R01 AR038648/AR/NIAMS NIH HHS/United States
GR  - NS33199/NS/NINDS NIH HHS/United States
GR  - R37 AR038648/AR/NIAMS NIH HHS/United States
GR  - R37 NS033199/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Cdkn1a protein, mouse)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Klf7 protein, mouse)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Axons/metabolism
MH  - Cell Cycle Proteins/genetics/metabolism
MH  - Cells, Cultured
MH  - Central Nervous System/*embryology/growth & development
MH  - Chromatin Immunoprecipitation
MH  - Cyclin-Dependent Kinase Inhibitor p21
MH  - DNA-Binding Proteins/*genetics/*physiology
MH  - Embryo Loss/genetics
MH  - Gene Expression Regulation, Developmental
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Kruppel-Like Transcription Factors
MH  - Mice
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - *Morphogenesis
MH  - Neurons/cytology/*metabolism
MH  - Olfactory Mucosa/cytology
MH  - Promoter Regions, Genetic
MH  - Retina/cytology
MH  - Tissue Distribution
MH  - Transcription Factors/*genetics/*physiology
MH  - Transcription, Genetic
PMC - PMC1157008
EDAT- 2005/06/21 09:00
MHDA- 2005/08/03 09:00
PMCR- 2005/11/01
CRDT- 2005/06/21 09:00
PHST- 2005/06/21 09:00 [pubmed]
PHST- 2005/08/03 09:00 [medline]
PHST- 2005/06/21 09:00 [entrez]
PHST- 2005/11/01 00:00 [pmc-release]
AID - 25/13/5699 [pii]
AID - 2485-04 [pii]
AID - 10.1128/MCB.25.13.5699-5711.2005 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2005 Jul;25(13):5699-711. doi: 10.1128/MCB.25.13.5699-5711.2005.