PMID- 15965067
OWN - NLM
STAT- MEDLINE
DCOM- 20060911
LR  - 20131121
IS  - 0077-8923 (Print)
IS  - 0077-8923 (Linking)
VI  - 1042
DP  - 2005 May
TI  - S-nitrosoglutathione and hypoxia-inducible factor-1 confer chemoresistance 
      against carbamoylating cytotoxicity of BCNU in rat C6 glioma cells.
PG  - 229-34
AB  - BCNU (1,3-bis[2-chloroethyl]-1-nitrosourea) is the mainstay in glioblastoma 
      multiform chemotherapy with only minimal effects. BCNU may kill tumor cells via 
      carbamoylating cytotoxicity, which irreversibly inhibits glutathione reductase 
      with resultant accumulation of oxidized form of glutathione causing oxidative 
      stress. S-nitrosoglutathione (GSNO) is a product of glutathione and nitric oxide 
      interaction. We report that GSNO formation may underlie carbamoylating 
      chemoresistance mediated by activation of inducible nitric oxide synthase. 
      Transactivation of hypoxia-inducible factor-1 (HIF-1)-responsive genes reduces 
      oxidative stress caused by glutathione depletion. We also noted that 
      preconditioning of C6 glioma cells to induce HIF-1 and its downstream genes 
      confers chemoresistance against carbamoylating cytotoxicity of BCNU.
FAU - Yang, Ding-I
AU  - Yang DI
AD  - Institute of Neuroscience, Tzu Chi University, Hualien, Taiwan.
FAU - Chen, Shang-Der
AU  - Chen SD
FAU - Yin, Jiu-Haw
AU  - Yin JH
FAU - Hsu, Chung Y
AU  - Hsu CY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 57564-91-7 (S-Nitrosoglutathione)
RN  - U68WG3173Y (Carmustine)
SB  - IM
MH  - Animals
MH  - Carmustine/*chemistry/*toxicity
MH  - Cell Line, Tumor
MH  - *Drug Resistance, Neoplasm
MH  - Glioma/*drug therapy/*metabolism/pathology
MH  - Hypoxia-Inducible Factor 1/*metabolism
MH  - Nitric Oxide/metabolism
MH  - Rats
MH  - S-Nitrosoglutathione/*metabolism
EDAT- 2005/06/21 09:00
MHDA- 2006/09/12 09:00
CRDT- 2005/06/21 09:00
PHST- 2005/06/21 09:00 [pubmed]
PHST- 2006/09/12 09:00 [medline]
PHST- 2005/06/21 09:00 [entrez]
AID - 1042/1/229 [pii]
AID - 10.1196/annals.1338.025 [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2005 May;1042:229-34. doi: 10.1196/annals.1338.025.