PMID- 15965612
OWN - NLM
STAT- MEDLINE
DCOM- 20090909
LR  - 20181113
IS  - 1572-994X (Electronic)
IS  - 0920-8569 (Linking)
VI  - 31
IP  - 1
DP  - 2005 Aug
TI  - Identification of a novel B cell epitope on the nucleocapsid protein of porcine 
      reproductive and respiratory syndrome virus by phage display.
PG  - 81-7
AB  - A phage display peptide library targeting the nucleocapsid (N) protein of porcine 
      reproductive and respiratory syndrome virus (PRRSV) strain CH-1a was generated 
      and used for epitope mapping. After 3 rounds of biopanning with the monoclonal 
      antibody (MAb) N3H2 directed against the N protein, 3 positive phages were 
      screened and sequenced. These phages share a consensus sequence, IQTAFNQGA, which 
      corresponds to the amino acid (AA) 79-87 segment of the CH-1a N protein. A small 
      DNA fragment coding for IQTAFNQGA was expressed as a fusion product, and reacted 
      to N3H2 in Western blots and indirect ELISA. Four truncated peptides (IQTAFNQG, 
      IQTAFNQ, QTAFNQGA, and TAFNQGA) expressed as GST fusion products failed to react 
      with N3H2. The sequences around the N3H2-binding site among the N proteins of 57 
      PRRSV strains were compared. Our results indicate that the IQTAFNQGA motif is 
      highly conserved among North American and European isolates. We concluded that 
      the precisely defined nona-peptide epitope is a novel conserved Linear B cell 
      epitope on the N protein of PRRSV.
FAU - An, Tong-Qing
AU  - An TQ
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, CAAS, 427 Maduan Street, 150001, Harbin, People's Republic of China.
FAU - Zhou, Yan-Jun
AU  - Zhou YJ
FAU - Qiu, Hua-Ji
AU  - Qiu HJ
FAU - Tong, Guang-Zhi
AU  - Tong GZ
FAU - Wang, Yun-Feng
AU  - Wang YF
FAU - Liu, Jin-Xia
AU  - Liu JX
FAU - Yang, Jin-Yu
AU  - Yang JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Virus Genes
JT  - Virus genes
JID - 8803967
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Peptide Library)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Epitopes, B-Lymphocyte/chemistry/*genetics/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - Nucleocapsid Proteins/chemistry/genetics/*immunology
MH  - *Peptide Library
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/chemistry/*genetics/*immunology
MH  - Sequence Alignment
EDAT- 2005/06/21 09:00
MHDA- 2009/09/10 06:00
CRDT- 2005/06/21 09:00
PHST- 2004/11/07 00:00 [received]
PHST- 2005/01/25 00:00 [accepted]
PHST- 2005/01/25 00:00 [revised]
PHST- 2005/06/21 09:00 [pubmed]
PHST- 2009/09/10 06:00 [medline]
PHST- 2005/06/21 09:00 [entrez]
AID - 10.1007/s11262-005-2203-1 [doi]
PST - ppublish
SO  - Virus Genes. 2005 Aug;31(1):81-7. doi: 10.1007/s11262-005-2203-1.