PMID- 1596571
OWN - NLM
STAT- MEDLINE
DCOM- 19920709
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 79
IP  - 12
DP  - 1992 Jun 15
TI  - Fluorescence in situ hybridization: a sensitive method for trisomy 8 detection in 
      bone marrow specimens.
PG  - 3307-15
AB  - Trisomy 8 is a common anomaly in bone marrow (BM) cells of patients with 
      myeloproliferative disorders (MPD), myelodysplastic syndromes (MDS), or acute 
      nonlymphocytic leukemia (ANLL). We studied the efficacy of fluorescence in situ 
      hybridization (FISH) detection of trisomy 8 in patients with MPD, MDS, or ANLL 
      using directly labeled fluorescent alpha-satellite and whole chromosome paint 
      (WCP) DNA probes specific for chromosome 8. Using FISH, we analyzed interphase 
      nuclei and metaphase spreads from randomized series of BM specimens from normal 
      individuals and patients with varying proportions of trisomy 8 as determined by 
      conventional cytogenetic analysis. The BM of all normal donors contained less 
      than or equal to 2.0% nuclei with 3 interphase FISH signals and less than or 
      equal to 1 metaphase with 3 WCP FISH signals. Ninety-five percent and 98% of BM 
      specimens with at least two metaphase cells with trisomy 8 by cytogenetic 
      analysis contained greater than 2.0% nuclei with 3 interphase FISH and greater 
      than 2 metaphases with 3 WCP FISH signals, respectively. Thirteen patients had 1 
      in 20 or 1 in 30 metaphase cells with trisomy 8 by conventional cytogenetic 
      studies. Of these patients, four had greater than 2.0% nuclei with 3 interphase 
      FISH signals. The BM of all four patients contained positive metaphase FISH 
      results. We then studied the usefulness of FISH analysis to detect occult trisomy 
      8 by analyzing BM nuclei from 144 patients who had MPD, MDS, or ANLL and either 
      20 normal metaphase cells or an abnormal karyotype without trisomy 8. Seven 
      patients had greater than 2.0% nuclei with 3 interphase FISH signals (range, 
      2.10% to 3.40%) and six patients had 2 or more cells with trisomy 8 upon 
      metaphase FISH or extensive conventional cytogenetic analysis. Our results show 
      that interphase and metaphase FISH analyses are useful methods to detect trisomy 
      8 cells in BM specimens, especially for specimens with normal or uncertain 
      conventional cytogenetic results.
FAU - Jenkins, R B
AU  - Jenkins RB
AD  - Section of Laboratory Genetics, Mayo Clinic and Foundation, Rochester, MN 55905.
FAU - Le Beau, M M
AU  - Le Beau MM
FAU - Kraker, W J
AU  - Kraker WJ
FAU - Borell, T J
AU  - Borell TJ
FAU - Stalboerger, P G
AU  - Stalboerger PG
FAU - Davis, E M
AU  - Davis EM
FAU - Penland, L
AU  - Penland L
FAU - Fernald, A
AU  - Fernald A
FAU - Espinosa, R 3rd
AU  - Espinosa R 3rd
FAU - Schaid, D J
AU  - Schaid DJ
AU  - et al.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Bone Marrow/*ultrastructure
MH  - DNA Probes
MH  - Fluorescent Dyes
MH  - Humans
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Myelodysplastic Syndromes/*genetics
MH  - Myeloproliferative Disorders/*genetics
MH  - *Nucleic Acid Hybridization
MH  - *Trisomy
EDAT- 1992/06/15 00:00
MHDA- 1992/06/15 00:01
CRDT- 1992/06/15 00:00
PHST- 1992/06/15 00:00 [pubmed]
PHST- 1992/06/15 00:01 [medline]
PHST- 1992/06/15 00:00 [entrez]
AID - S0006-4971(20)71190-0 [pii]
PST - ppublish
SO  - Blood. 1992 Jun 15;79(12):3307-15.