PMID- 15965904
OWN - NLM
STAT- MEDLINE
DCOM- 20060317
LR  - 20051103
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 206
IP  - 1
DP  - 2006 Jan
TI  - TP53 mutations and S-phase fraction but not DNA-ploidy are independent prognostic 
      indicators in laryngeal squamous cell carcinoma.
PG  - 181-8
AB  - To prospectively evaluate the prognostic significance of TP53, H-, K-, and N-Ras 
      mutations, DNA-ploidy and S-phase fraction (SPF) in patients affected by locally 
      advanced laryngeal squamous cell carcinoma (LSCC). Eight-one patients (median 
      follow-up was 71 months) who underwent resective surgery for primary operable 
      locally advanced LSCC were analyzed. Tumor DNA was screened for mutational 
      analysis by PCR/SSCP and sequencing. DNA-ploidy and SPF were performed by flow 
      cytometric analyses. Thirty-six patients (44%) had, at least, a mutation in the 
      TP53 gene. Of them, 22% (8/36) had double mutations and 3% (1/36) had triple 
      mutations. In total, 46 TP53 mutations were observed. The majority (41%) of these 
      occur in exon 5 (19/46), while the mutations in exons 6, 7, and 8 were 
      represented in 14, 7, and 6 patients, respectively (31%, 15%, and 16%). Five LSCC 
      patients (6%) showed a mutation in H-Ras gene. Sixty-three percent of the cases 
      (51/81) were DNA aneuploidy, 14% of these (7/51) were multiclonal. Thirty-nine 
      patients (48%) had an high SPF value. At Univariate analysis, the DNA aneuploidy, 
      high SPF (>15.1%), TP53 mutations and, in particular, the mutations that occur in 
      exons 5 and 8 were significantly related to quicker disease relapse and short OS. 
      At Multivariate analysis, the major significant predictors for both disease 
      relapse and death were high SPF and any TP53 mutations. While histological grade 
      G3 was an independent factor only for relapse. In conclusions, any TP53 mutations 
      and high SPF are important biological indicators to predict the outcome of LSCC 
      patients.
CI  - Copyright 2005 Wiley-Liss, Inc.
FAU - Russo, Antonio
AU  - Russo A
AD  - Section and Oncology, Department of Oncology, Universita di Palermo, Palermo, 
      Italy. lab-oncobiologia@usa.net
FAU - Corsale, Simona
AU  - Corsale S
FAU - Agnese, Valentina
AU  - Agnese V
FAU - Macaluso, Marcella
AU  - Macaluso M
FAU - Cascio, Sandra
AU  - Cascio S
FAU - Bruno, Loredana
AU  - Bruno L
FAU - Surmacz, Eva
AU  - Surmacz E
FAU - Dardanoni, Gabriella
AU  - Dardanoni G
FAU - Valerio, Maria Rosaria
AU  - Valerio MR
FAU - Vieni, Salvatore
AU  - Vieni S
FAU - Restivo, Salvatore
AU  - Restivo S
FAU - Fulfaro, Fabio
AU  - Fulfaro F
FAU - Tomasino, Rosa Maria
AU  - Tomasino RM
FAU - Gebbia, Nicola
AU  - Gebbia N
FAU - Bazan, Viviana
AU  - Bazan V
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Carcinoma, Squamous Cell/*diagnosis/*genetics/pathology
MH  - DNA Mutational Analysis
MH  - DNA, Neoplasm
MH  - Genes, ras
MH  - Humans
MH  - Laryngeal Neoplasms/*diagnosis/*genetics/pathology
MH  - Mutation
MH  - *Ploidies
MH  - Polymorphism, Single-Stranded Conformational
MH  - Prognosis
MH  - S Phase/*physiology
MH  - Survival Rate
MH  - Tumor Suppressor Protein p53/*genetics
EDAT- 2005/06/21 09:00
MHDA- 2006/03/18 09:00
CRDT- 2005/06/21 09:00
PHST- 2005/06/21 09:00 [pubmed]
PHST- 2006/03/18 09:00 [medline]
PHST- 2005/06/21 09:00 [entrez]
AID - 10.1002/jcp.20447 [doi]
PST - ppublish
SO  - J Cell Physiol. 2006 Jan;206(1):181-8. doi: 10.1002/jcp.20447.