PMID- 15967530
OWN - NLM
STAT- MEDLINE
DCOM- 20051013
LR  - 20161124
IS  - 0168-1605 (Print)
IS  - 0168-1605 (Linking)
VI  - 103
IP  - 3
DP  - 2005 Sep 15
TI  - Multiple norovirus genotypes characterised from an oyster-associated outbreak of 
      gastroenteritis.
PG  - 323-30
AB  - The diversity of norovirus (NV) genotypes was investigated in persons who were 
      ill with acute gastroenteritis associated with the consumption of oysters. 
      Initial results from a commercial enzyme immunoassay (EIA) indicated a mixed NV 
      genogroup I (GI) and II (GII) outbreak. A reverse-transcriptase (RT)-PCR for NVs 
      was applied to nucleic acid extracted from faecal specimens collected from 
      symptomatic cases. Using primers that amplified contiguous sequences in the 
      ORF1/2 region of the NV genome and a hemi-nested PCR derived from this assay, 
      three different GII and two GI NV genotypes were detected and the strains were 
      characterised by DNA sequencing. Using this approach a recombinant NV genotype, 
      rGII-3a (recombinant Harrow/Mexico) the predominant strain identified in several 
      symptomatic cases from the outbreak, was detected and characterised. No other 
      gastroenteric viruses, including rotavirus, astrovirus, sapovirus and adenovirus 
      40/41 were detected by RT-PCR and PCR.
FAU - Gallimore, Chris I
AU  - Gallimore CI
AD  - Enteric, Respiratory and Neurological Virus Laboratory, Specialist and Reference 
      Microbiology Division, Health Protection Agency Centre for Infections, Colindale, 
      London, NW9 5HT, United Kingdom. christopher.gallimore@hpa.org.uk
FAU - Cheesbrough, John S
AU  - Cheesbrough JS
FAU - Lamden, Kenneth
AU  - Lamden K
FAU - Bingham, Chris
AU  - Bingham C
FAU - Gray, Jim J
AU  - Gray JJ
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Int J Food Microbiol
JT  - International journal of food microbiology
JID - 8412849
SB  - IM
MH  - Animals
MH  - Caliciviridae Infections/*epidemiology/virology
MH  - Consumer Product Safety
MH  - Disease Outbreaks
MH  - Food Contamination/analysis
MH  - Gastroenteritis/*epidemiology/*virology
MH  - Genotype
MH  - Humans
MH  - *Norovirus/classification/isolation & purification
MH  - Ostreidae/*virology
MH  - Phylogeny
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Shellfish/*virology
MH  - United Kingdom/epidemiology
EDAT- 2005/06/22 09:00
MHDA- 2005/10/14 09:00
CRDT- 2005/06/22 09:00
PHST- 2005/02/20 00:00 [accepted]
PHST- 2005/06/22 09:00 [pubmed]
PHST- 2005/10/14 09:00 [medline]
PHST- 2005/06/22 09:00 [entrez]
AID - S0168-1605(05)00165-0 [pii]
AID - 10.1016/j.ijfoodmicro.2005.02.003 [doi]
PST - ppublish
SO  - Int J Food Microbiol. 2005 Sep 15;103(3):323-30. doi: 
      10.1016/j.ijfoodmicro.2005.02.003.