PMID- 15967781
OWN - NLM
STAT- MEDLINE
DCOM- 20051223
LR  - 20061115
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 17
IP  - 7
DP  - 2005 Jul
TI  - GATA-1 is required for expression of FcepsilonRI on mast cells: analysis of 
      mast cells derived from GATA-1 knockdown mouse bone marrow.
PG  - 847-56
AB  - The high-affinity receptor for IgE (FcepsilonRI) that is expressed on the surface 
      of mast cells plays an important role in antigen/IgE-mediated allergic reactions. 
      We have previously found that critical elements in the promoter of the 
      FcepsilonRI alpha- and beta-chain genes are recognized by the transcription 
      factor GATA-1 in electrophoretic mobility shift assays coupled with a transient 
      expression system for the alpha- and beta-chain promoters. To confirm that GATA-1 
      is involved in the expression of FcepsilonRI definitively, we generated bone 
      marrow-derived mast cells from GATA-1 knockdown (KD) heterozygous mice. FACS 
      analysis showed that the frequency of FcepsilonRI-positive cells was 
      significantly decreased in mast cells derived from bone marrow of GATA-1 KD mice. 
      Reverse transcription-PCR analysis showed that the level of transcripts not only 
      for GATA-1 but also for both the alpha- and beta-chains was significantly lower 
      in KD mast cells, whereas that of the FcepsilonRI gamma-chain was not affected. 
      Degranulation caused by cross-linking of FcepsilonRI on mast cells prepared from 
      KD mice was markedly repressed in comparison with that of wild-type mast cells. 
      We concluded that the transcription factor GATA-1 positively regulates 
      FcepsilonRI alpha- and beta-chain expression and therefore is involved in mast 
      cell development.
FAU - Nishiyama, Chiharu
AU  - Nishiyama C
AD  - Atopy (Allergy) Research Center, Juntendo University School of Medicine, 2-1-1 
      Hongo, Bunkyo-ku, Tokyo 113-8421, Japan. chinishi@med.juntendo.ac.jp
FAU - Ito, Tomonobu
AU  - Ito T
FAU - Nishiyama, Makoto
AU  - Nishiyama M
FAU - Masaki, Shigehiro
AU  - Masaki S
FAU - Maeda, Keiko
AU  - Maeda K
FAU - Nakano, Nobuhiro
AU  - Nakano N
FAU - Ng, William
AU  - Ng W
FAU - Fukuyama, Kanako
AU  - Fukuyama K
FAU - Yamamoto, Masayuki
AU  - Yamamoto M
FAU - Okumura, Ko
AU  - Okumura K
FAU - Ogawa, Hideoki
AU  - Ogawa H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050620
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Receptors, IgE)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*immunology
MH  - Cell Degranulation/genetics/*immunology
MH  - Cell Differentiation/genetics/immunology
MH  - Female
MH  - Gene Expression Regulation/genetics/immunology
MH  - Mast Cells/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Receptors, IgE/genetics/*immunology
EDAT- 2005/06/22 09:00
MHDA- 2005/12/24 09:00
CRDT- 2005/06/22 09:00
PHST- 2005/06/22 09:00 [pubmed]
PHST- 2005/12/24 09:00 [medline]
PHST- 2005/06/22 09:00 [entrez]
AID - dxh278 [pii]
AID - 10.1093/intimm/dxh278 [doi]
PST - ppublish
SO  - Int Immunol. 2005 Jul;17(7):847-56. doi: 10.1093/intimm/dxh278. Epub 2005 Jun 20.