PMID- 15967828
OWN - NLM
STAT- MEDLINE
DCOM- 20050824
LR  - 20220409
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 201
IP  - 12
DP  - 2005 Jun 20
TI  - Myeloid and plasmacytoid dendritic cells transfer HIV-1 preferentially to 
      antigen-specific CD4+ T cells.
PG  - 2023-33
AB  - Dendritic cells (DCs) are essential antigen-presenting cells for the induction of 
      T cell immunity against pathogens such as human immunodeficiency virus (HIV)-1. 
      At the same time, HIV-1 replication is strongly enhanced in DC-T cell clusters, 
      potentially undermining this process. We found that immature CD123(+) 
      plasmacytoid DCs (PDCs) and CD11c(+) myeloid DCs (MDCs) were susceptible to both 
      a CCR5- and a CXCR4-using HIV-1 isolate in vitro and were able to efficiently 
      transfer that infection to autologous CD4(+) T cells. Soon after HIV-1 exposure, 
      both PDCs and MDCs were able to transfer the virus to T cells in the absence of a 
      productive infection. However, once a productive infection was established in the 
      DCs, newly synthesized virus was predominantly spread to T cells. HIV-1 exposure 
      of the MDCs and PDCs did not inhibit their ability to present cytomegalovirus 
      (CMV) antigens and activate CMV-specific memory T cells. As a result, both PDCs 
      and MDCs preferentially transmitted HIV-1 to the responding CMV antigen-specific 
      CD4(+) T cells rather than to nonresponding T cells. This suggests that the 
      induction of antigen-specific T cell responses by DCs, a process crucial to 
      immune defense, can lead to preferential HIV-1 infection and the deletion of 
      responding CD4(+) T cells.
FAU - Lore, Karin
AU  - Lore K
AD  - Immunology Laboratory, Vaccine Research Center, National Institute of Allergy and 
      Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA. 
      klore@mail.nih.gov
FAU - Smed-Sorensen, Anna
AU  - Smed-Sorensen A
FAU - Vasudevan, Jayanand
AU  - Vasudevan J
FAU - Mascola, John R
AU  - Mascola JR
FAU - Koup, Richard A
AU  - Koup RA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (5-(6)-carboxyfluorescein diacetate succinimidyl ester)
RN  - 0 (Antigens, Viral)
RN  - 0 (CD11 Antigens)
RN  - 0 (DNA Primers)
RN  - 0 (Fluoresceins)
RN  - 0 (IL3RA protein, human)
RN  - 0 (Interferon-alpha)
RN  - 0 (Interleukin-3 Receptor alpha Subunit)
RN  - 0 (Receptors, CCR5)
RN  - 0 (Receptors, CXCR4)
RN  - 0 (Receptors, Interleukin-3)
RN  - 0 (Succinimides)
SB  - IM
MH  - Antigen Presentation/*immunology
MH  - Antigens, Viral/immunology/metabolism
MH  - CD11 Antigens/immunology
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Cytomegalovirus/immunology
MH  - DNA Primers
MH  - Dendritic Cells/*immunology/*virology
MH  - Flow Cytometry
MH  - Fluoresceins
MH  - HIV-1/genetics/*immunology
MH  - Humans
MH  - Interferon-alpha/metabolism
MH  - Interleukin-3 Receptor alpha Subunit
MH  - Polymerase Chain Reaction
MH  - Receptors, CCR5/immunology/metabolism
MH  - Receptors, CXCR4/immunology/metabolism
MH  - Receptors, Interleukin-3/immunology
MH  - Succinimides
PMC - PMC2212038
EDAT- 2005/06/22 09:00
MHDA- 2005/08/25 09:00
PMCR- 2005/12/20
CRDT- 2005/06/22 09:00
PHST- 2005/06/22 09:00 [pubmed]
PHST- 2005/08/25 09:00 [medline]
PHST- 2005/06/22 09:00 [entrez]
PHST- 2005/12/20 00:00 [pmc-release]
AID - jem.20042413 [pii]
AID - 20042413 [pii]
AID - 10.1084/jem.20042413 [doi]
PST - ppublish
SO  - J Exp Med. 2005 Jun 20;201(12):2023-33. doi: 10.1084/jem.20042413.