PMID- 15969687
OWN - NLM
STAT- MEDLINE
DCOM- 20051208
LR  - 20131121
IS  - 0105-4538 (Print)
IS  - 0105-4538 (Linking)
VI  - 60
IP  - 8
DP  - 2005 Aug
TI  - Relationships between specific serum IgE, cytokines and polymorphisms in the 
      IL-4, IL-4Ralpha in patients with penicillins allergy.
PG  - 1053-9
AB  - BACKGROUND: Excessive production of interleukin (IL)-4, IL-13 and interferon 
      (IFN)-gamma is thought to be important in the development of allergic disease and 
      atopy. Several investigators have linked the IL-4 and IL-4R genes to allergic 
      disease and atopy. The aim of this study is to further explore the mechanism of 
      penicillins allergy and evaluate the possible role of the IL-4 C-589T and 
      IL-4RalphaQ576R polymorphisms in modulating the allergic responses to 
      penicillins. METHODS: Radioallergosorbent test (RAST) was used to detect eight 
      kinds of specific immunoglobulin E (IgE) to penicillins in serum. Serum levels of 
      IL-4, IL-13 and IFN-gamma were measured by using enzyme-linked immunosorbent 
      assay (ELISA). The IL-4 C-589T and IL-4RalphaQ576R polymorphisms were genotyped 
      by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). 
      RESULTS: Compared with control subjects, there were significantly higher levels 
      of IL-4, IL-13 and IFN-gamma in allergic patients with positive specific IgE (P < 
      0.01), and the lower levels of IL-4 and IFN-gamma were observed in allergic 
      patients with negative specific IgE (P < 0.05). We found a growing trend of IL-4 
      and IL-13 levels with the kind increasing of positive specific IgE, and even 
      there were significant correlations between the three kinds of cytokines and many 
      kinds of specific IgE (P < 0.05). The IL-4Ralpha*Q576 allele was significantly 
      increased in patients with penicillins allergy compared with control subjects (P 
      < 0.01). Furthermore, the allele was strongly associated with increased 
      serum-specific benzylpenicilloyl (BPO)-, phenoxomethylpenicillanyl (PVA)- or 
      ampicillanyl (APA)-IgE levels in patients with positive specific IgE (P < 0.05). 
      CONCLUSIONS: These data suggest that IL-4, IL-13 and IFN-gamma play an important 
      roles in penicillins allergy. The IL-4RalphaQ576R polymorphism may involve in the 
      development of penicillins allergy, and through modulating specific serum IgE 
      levels.
FAU - Qiao, H-L
AU  - Qiao HL
AD  - Department of Clinical Pharmacology, School of Medicine, Zhengzhou University, 
      Zhengzhou, China.
FAU - Yang, J
AU  - Yang J
FAU - Zhang, Y-W
AU  - Zhang YW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Cytokines)
RN  - 0 (IL4R protein, human)
RN  - 0 (Interleukin-13)
RN  - 0 (Interleukin-4 Receptor alpha Subunit)
RN  - 0 (Penicillins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Cell Surface)
RN  - 0RH81L854J (Glutamine)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 94ZLA3W45F (Arginine)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Arginine
MH  - Child
MH  - Cytokines/*blood
MH  - Drug Hypersensitivity/*blood/etiology/*genetics
MH  - Female
MH  - Glutamine
MH  - Humans
MH  - Immunoglobulin E/*metabolism
MH  - Interferon-gamma/blood
MH  - Interleukin-13/blood
MH  - Interleukin-4/blood/*genetics
MH  - Interleukin-4 Receptor alpha Subunit
MH  - Male
MH  - Middle Aged
MH  - Penicillins/*adverse effects
MH  - Protein Isoforms/genetics
MH  - Receptors, Cell Surface/*genetics
EDAT- 2005/06/23 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/06/23 09:00
PHST- 2005/06/23 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/06/23 09:00 [entrez]
AID - ALL816 [pii]
AID - 10.1111/j.1398-9995.2005.00816.x [doi]
PST - ppublish
SO  - Allergy. 2005 Aug;60(8):1053-9. doi: 10.1111/j.1398-9995.2005.00816.x.