PMID- 15970637
OWN - NLM
STAT- MEDLINE
DCOM- 20050927
LR  - 20240109
IS  - 1018-2438 (Print)
IS  - 1018-2438 (Linking)
VI  - 137
IP  - 4
DP  - 2005 Aug
TI  - Suppression of Th2 immune responses by mekabu fucoidan from Undaria pinnatifida 
      sporophylls.
PG  - 289-94
AB  - BACKGROUND: We demonstrated that mekabu fucoidan obtained from Undaria 
      pinnatifida (Up) sporophylls augments the type 1 T-helper (Th1) cell response in 
      normal BALB/c mice. In this study, we examined the effects of the fucoidan of 
      mekabu on the type 2 T-helper (Th2) response in bronchoalveolar lavage fluid 
      (BALF) after ovalbumin (OVA) aerosol challenge. METHODS: Mekabu fucoidan (50 
      mg/kg) was injected intraperitoneally into BALB/c mice for 4 days, and then the 
      mice were sensitized with 50 microg/mouse of OVA plus alum (1 mg/mouse) 1 and 8 
      days later. The mice were challenged with OVA delivered using a nebulizer 7, 8 
      and 9 days after the second challenge with OVA plus alum. After 24 h, we assessed 
      T cell responses in BALF by measuring the amount of Th2 cytokines (IL-4, IL-5, 
      IL-13) and gamma-interferon (IFN-gamma) produced by Th1 cells. RESULTS: The 
      production of Th2 cytokines was suppressed (p < 0.05), and the amount of 
      IFN-gamma was not increased in the mice treated with mekabu fucoidan. Anti-OVA 
      immunoglobulin E (IgE) and IgE levels in serum determined after challenge with 
      aerosolized OVA at the end of the experiment were lower (p < 0.05) in the treated 
      than in the control mice. CONCLUSIONS: The pulmonary inflammation was relieved by 
      mekabu fucoidan, which also downregulated Th2-dominated responses. These results 
      indicate that mekabu fucoidan modulates Th2 responses and might be useful for 
      treating allergic inflammation.
FAU - Maruyama, Hiroko
AU  - Maruyama H
AD  - Department of Pathology, School of Allied Health Sciences, Kitasato University, 
      Sagamihara, Japan. maruyama@ahs.kitasato-u.ac.jp
FAU - Tamauchi, Hidekazu
AU  - Tamauchi H
FAU - Hashimoto, Minoru
AU  - Hashimoto M
FAU - Nakano, Takahisa
AU  - Nakano T
LA  - eng
PT  - Journal Article
DEP - 20050617
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Polysaccharides)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
RN  - 9072-19-9 (fucoidan)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/administration & dosage/*immunology
MH  - Bronchial Provocation Tests
MH  - Bronchoalveolar Lavage Fluid/immunology
MH  - Cytokines/immunology
MH  - Immunoglobulin E/immunology
MH  - Injections, Intraperitoneal
MH  - Interferon-gamma/immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Models, Animal
MH  - Ovalbumin/adverse effects
MH  - Pneumonia/*immunology
MH  - Polysaccharides/administration & dosage/*immunology
MH  - Th2 Cells/*immunology
MH  - Undaria/*immunology
EDAT- 2005/06/23 09:00
MHDA- 2005/09/28 09:00
CRDT- 2005/06/23 09:00
PHST- 2004/09/21 00:00 [received]
PHST- 2005/02/21 00:00 [accepted]
PHST- 2005/06/23 09:00 [pubmed]
PHST- 2005/09/28 09:00 [medline]
PHST- 2005/06/23 09:00 [entrez]
AID - 86422 [pii]
AID - 10.1159/000086422 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2005 Aug;137(4):289-94. doi: 10.1159/000086422. Epub 
      2005 Jun 17.