PMID- 15972641
OWN - NLM
STAT- MEDLINE
DCOM- 20050831
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 175
IP  - 1
DP  - 2005 Jul 1
TI  - Independent trafficking of Ig-alpha/Ig-beta and mu-heavy chain is facilitated by 
      dissociation of the B cell antigen receptor complex.
PG  - 147-54
AB  - The BCR relays extracellular signals and internalizes Ag for processing and 
      presentation. We have previously demonstrated that ligation of the BCR 
      destabilizes Ig-alpha/Ig-beta (Ig-alphabeta) from mu-H chain (mum). In this study 
      we report that receptor destabilization represents a physical separation of mum 
      from Ig-alphabeta. Sucrose gradient fractionation localized Ig-alphabeta to 
      G(M1)-containing lipid microdomains in the absence of mum. Confocal and electron 
      microscopy studies revealed the colocalization of unsheathed mum with 
      clathrin-coated vesicles. Furthermore, mum failed to associate with 
      clathrin-coated vesicles when receptor destabilization was inhibited, suggesting 
      that unsheathing of mum is required for clathrin-mediated endocytosis. In 
      summary, we found that Ag stimulation physically separates Ig-alphabeta from mum, 
      facilitating concomitant signal transduction and Ag delivery to the endocytic 
      compartment.
FAU - Kim, Jin-Hyang
AU  - Kim JH
AD  - Department of Pathology and the Cancer Research and Treatment Center, University 
      of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA.
FAU - Cramer, Lorraine
AU  - Cramer L
FAU - Mueller, Heather
AU  - Mueller H
FAU - Wilson, Bridget
AU  - Wilson B
FAU - Vilen, Barbara J
AU  - Vilen BJ
LA  - eng
GR  - R01 AI053266/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Immunoglobulin alpha-Chains)
RN  - 0 (Immunoglobulin mu-Chains)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 37758-47-7 (G(M1) Ganglioside)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Biological Transport, Active
MH  - Cell Line
MH  - Clathrin-Coated Vesicles/immunology/metabolism
MH  - Endocytosis
MH  - G(M1) Ganglioside/metabolism
MH  - Immunoglobulin Heavy Chains/*metabolism
MH  - Immunoglobulin alpha-Chains/metabolism
MH  - Immunoglobulin mu-Chains/metabolism
MH  - Kinetics
MH  - Membrane Microdomains/immunology/metabolism
MH  - Mice
MH  - Microscopy, Electron
MH  - Receptors, Antigen, B-Cell/*metabolism
MH  - Signal Transduction
PMC - PMC3895480
MID - NIHMS541643
COIS- Disclosures The authors have no financial conflict of interest.
EDAT- 2005/06/24 09:00
MHDA- 2005/09/01 09:00
PMCR- 2014/01/19
CRDT- 2005/06/24 09:00
PHST- 2005/06/24 09:00 [pubmed]
PHST- 2005/09/01 09:00 [medline]
PHST- 2005/06/24 09:00 [entrez]
PHST- 2014/01/19 00:00 [pmc-release]
AID - 175/1/147 [pii]
AID - 10.4049/jimmunol.175.1.147 [doi]
PST - ppublish
SO  - J Immunol. 2005 Jul 1;175(1):147-54. doi: 10.4049/jimmunol.175.1.147.