PMID- 1597505
OWN - NLM
STAT- MEDLINE
DCOM- 19920709
LR  - 20190827
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 37
IP  - 2
DP  - 1992 May
TI  - A modified alkaline phosphatase enzyme amplification system and its application 
      in an HIV antigen ELISA.
PG  - 149-53
AB  - Alkaline phosphatase amplification systems increase the sensitivity of 
      enzyme-linked immunoassays (ELISAs). Here we describe a modified method by which 
      levamisole is a component of the substrate buffer. This increases sensitivity by 
      reducing the signal from non-specific alkaline phosphatases. The modified 
      procedure was applied to an 'in house' HIV-1 p24 antigen capture assay and was 
      shown to increase the sensitivity 4-fold as compared to the unmodified system. 
      The performance of the modified amplification system is comparable to that of 
      commercially available systems.
FAU - Ball, J K
AU  - Ball JK
AD  - Regional Virus Laboratory, East Birmingham Hospital, U.K.
FAU - Beards, G M
AU  - Beards GM
FAU - Desselberger, U
AU  - Desselberger U
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (HIV Core Protein p24)
RN  - 2880D3468G (Levamisole)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - *Alkaline Phosphatase/standards
MH  - *Enzyme-Linked Immunosorbent Assay/standards
MH  - HIV Core Protein p24/*analysis
MH  - Levamisole
MH  - Substrate Specificity
EDAT- 1992/05/01 00:00
MHDA- 1992/05/01 00:01
CRDT- 1992/05/01 00:00
PHST- 1992/05/01 00:00 [pubmed]
PHST- 1992/05/01 00:01 [medline]
PHST- 1992/05/01 00:00 [entrez]
AID - 0166-0934(92)90042-C [pii]
AID - 10.1016/0166-0934(92)90042-c [doi]
PST - ppublish
SO  - J Virol Methods. 1992 May;37(2):149-53. doi: 10.1016/0166-0934(92)90042-c.