PMID- 15975275
OWN - NLM
STAT- MEDLINE
DCOM- 20070907
LR  - 20050624
IS  - 1007-3418 (Print)
IS  - 1007-3418 (Linking)
VI  - 13
IP  - 6
DP  - 2005 Jun
TI  - [An in vivo study on interlukin-10 inhibiting the expression and secretion of 
      collagen I and IV in hepatic stellate cells].
PG  - 425-7
AB  - OBJECTIVES: To investigate the effect of interlukin-10 (IL-10) on expression and 
      secretion of collagen I, IV in rat's hepatic stellate cells (HSC) of livers 
      injured by CCl4. METHOD: The adenovirus vector encoded IL-10 gene was used to 
      transfect rats with liver injury via the caudal veins. HSC were isolated and 
      purified from the rat livers by collagenase IV perfusion and density gradient 
      centrifugation with Nycodenz. The expression of collagen I, IV mRNA in HSC was 
      detected by semi-quantitative RT-PCR method and the secretion of collagen I, IV 
      in culture serum of HSC by ELISA method. The quantity of collagen was measured in 
      the van Gieson stained histological liver preparations. RESULTS: The expression 
      and secretion of collagen I, IV in the adenovirus vector encoding IL-10 gene 
      group were significantly lower than those in the adenovirus vector without IL-10 
      gene group and the control group (P < 0.05). The quantity of collagen in the 
      treatment group was lower than that in the control group. CONCLUSION: IL-10 can 
      inhibit collagen I, IV expression and secretion in rat HSC.
FAU - Xiong, Liang-fa
AU  - Xiong LF
AD  - Department of Hepatobiliary Surgery, People's Hospital, Peking University, 
      Beijing 100044, China.
FAU - Leng, Xi-sheng
AU  - Leng XS
FAU - Li, Tao
AU  - Li T
FAU - Wei, Yu-hua
AU  - Wei YH
FAU - Peng, Ji-run
AU  - Peng JR
FAU - Guo, Yan-tong
AU  - Guo YT
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Gan Zang Bing Za Zhi
JT  - Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of 
      hepatology
JID - 9710009
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type IV)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Collagen Type I/*biosynthesis/genetics
MH  - Collagen Type IV/*biosynthesis/genetics
MH  - Hepatocytes/*metabolism/pathology
MH  - Interleukin-10/*pharmacology
MH  - Liver Cirrhosis, Experimental/metabolism/*pathology
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2005/06/25 09:00
MHDA- 2007/09/08 09:00
CRDT- 2005/06/25 09:00
PHST- 2005/06/25 09:00 [pubmed]
PHST- 2007/09/08 09:00 [medline]
PHST- 2005/06/25 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Gan Zang Bing Za Zhi. 2005 Jun;13(6):425-7.