PMID- 15975306
OWN - NLM
STAT- MEDLINE
DCOM- 20050808
LR  - 20191210
IS  - 1672-0415 (Print)
IS  - 1672-0415 (Linking)
VI  - 11
IP  - 1
DP  - 2005 Mar
TI  - Study on the therapeutic mechanism of the active principle of the Chinese drug 
      Paeoniae Radix 801 through affinity biosensors IAsys plus quartz crystal 
      microbalance.
PG  - 37-40
AB  - OBJECTIVE: To study the targeted point and mechanism of the function of the 
      blood-activating and stasis-removing Chinese drugs, Paeoniae Radix 801(PR801) in 
      its cardiovascular protective effects and its specific binding with endothelin 1 
      (ET-1) as well as the dynamics of the two's interactive function by means of 
      using affinity biosensors: IAsys Plus and quartz crystal microbalance (IAQCM). 
      METHODS: ET-1 was immobilized on the surfaces of IAQCM by using the new surface 
      modification methods. The PR801 in the solution was detected by modified 
      substrates and the specific binding between PR801 and ET-1 was studied. RESULTS: 
      The curves went up or down after adding PR801. There is specific binding between 
      PR801 and ET-1. The bound mass were 0.458 ng/mm(2) and 133.54 ng/cm(2), 
      respectively. There exists relatively good stability with these two methods. 
      CONCLUSION: The affinity biosensors: IAQCM can be used to study the interaction 
      mechanism between PR801 and ET-1, providing a new way to study the interaction 
      mechanism of TCM. PR801 can bind ET-1 specifically in the experiments. Therefore, 
      ET-1 is another target that PR801 can bind specifically besides thromboxane A(2).
FAU - Huang, Jia-dong
AU  - Huang JD
AD  - College of Life Sciences, Nankai University, Tianjin, China.
FAU - Song, Zhao
AU  - Song Z
FAU - Li, Jing
AU  - Li J
FAU - Wu, Bao-yan
AU  - Wu BY
FAU - Yin, Hui-jun
AU  - Yin HJ
FAU - Chen, Ke-ji
AU  - Chen KJ
FAU - Chen, Qiang
AU  - Chen Q
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - China
TA  - Chin J Integr Med
JT  - Chinese journal of integrative medicine
JID - 101181180
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Endothelin-1)
RN  - 14808-60-7 (Quartz)
SB  - IM
MH  - *Biosensing Techniques/standards
MH  - Blood Circulation/drug effects
MH  - Drugs, Chinese Herbal/*metabolism/therapeutic use
MH  - Endothelin-1/*metabolism
MH  - Humans
MH  - Quartz
EDAT- 2005/06/25 09:00
MHDA- 2005/08/09 09:00
CRDT- 2005/06/25 09:00
PHST- 2005/06/25 09:00 [pubmed]
PHST- 2005/08/09 09:00 [medline]
PHST- 2005/06/25 09:00 [entrez]
AID - 10.1007/BF02835747 [doi]
PST - ppublish
SO  - Chin J Integr Med. 2005 Mar;11(1):37-40. doi: 10.1007/BF02835747.