PMID- 15976097
OWN - NLM
STAT- MEDLINE
DCOM- 20050816
LR  - 20190722
IS  - 0009-9147 (Print)
IS  - 0009-9147 (Linking)
VI  - 51
IP  - 7
DP  - 2005 Jul
TI  - Multiplexed analysis of biomarkers related to obesity and the metabolic syndrome 
      in human plasma, using the Luminex-100 system.
PG  - 1102-9
AB  - BACKGROUND: The complex pathology of disease has sparked the development of novel 
      protein expression profiling techniques that require validation in clinical 
      settings. This study focuses on multiplexed analyses of adipocytokines and 
      biomarkers linked to the metabolic syndrome, diabetes, and cardiovascular 
      disease. METHODS: Multiplexed immunoassays using fluorescent microspheres and the 
      Luminex-100 system were performed on plasma from 80 obese patients (40 with the 
      metabolic syndrome) before and after 6-8 weeks of diet-induced weight loss. 
      Leptin, insulin, C-peptide, monocyte chemoattractant protein-1 (MCP-1), eotaxin, 
      interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha), and IL-6 
      concentrations measured with multiplex panels from 3 different manufacturers were 
      compared with results from commercial ELISAs. Detection limits and between- and 
      within-run imprecision were determined for each analyte. Bland-Altman analysis 
      was used to determine agreement between multiplexed immunoassays and ELISAs. 
      RESULTS: Correlation between the Luminex multiplexed assays and ELISAs was good 
      for leptin (Linco), insulin (Linco), MCP-1 (Biosource and Upstate), and eotaxin 
      (Biosource) with correlation coefficients of 0.711-0.895; fair for eotaxin 
      (Upstate) and C-peptide (Linco) with correlation coefficients of 0.496-0.582; and 
      poor for TNF-alpha, IL-8, and IL-6 (Linco, Biosource, Upstate, and R&D) with 
      correlation coefficients of -0.107 to 0.318. Within- and between-run imprecision 
      values for the multiplex method were generally <15%. Relative changes in plasma 
      leptin and insulin concentrations after diet-induced weight loss were similar 
      whether assessed by multiplex assay or ELISA. CONCLUSION: Although this 
      technology appears useful in clinical research studies, low assay sensitivity and 
      poor correlations with conventional ELISA methods for some analytes with very low 
      plasma concentrations should be considered when using the Luminex platform in 
      clinical studies.
FAU - Liu, Mine Y
AU  - Liu MY
AD  - Section of Atherosclerosis, Department of Medicine, Baylor College of Medicine, 
      Houston, TX 77030, USA.
FAU - Xydakis, Antonios M
AU  - Xydakis AM
FAU - Hoogeveen, Ron C
AU  - Hoogeveen RC
FAU - Jones, Peter H
AU  - Jones PH
FAU - Smith, E O'Brian
AU  - Smith EO
FAU - Nelson, Kathleen W
AU  - Nelson KW
FAU - Ballantyne, Christie M
AU  - Ballantyne CM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Chem
JT  - Clinical chemistry
JID - 9421549
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Insulin)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Leptin)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adipose Tissue/metabolism
MH  - Biomarkers/blood
MH  - Cardiovascular Diseases/diagnosis
MH  - Chemokine CCL2/blood
MH  - Cytokines/blood
MH  - Female
MH  - Fluorescent Dyes
MH  - Humans
MH  - Immunoassay
MH  - Insulin/blood
MH  - Interleukin-6/blood
MH  - Interleukin-8/blood
MH  - Leptin/blood
MH  - Male
MH  - Metabolic Syndrome/*diagnosis
MH  - Microspheres
MH  - Middle Aged
MH  - Obesity/*diagnosis
MH  - Risk Factors
MH  - Tumor Necrosis Factor-alpha/analysis
MH  - Weight Loss
EDAT- 2005/06/25 09:00
MHDA- 2005/08/17 09:00
CRDT- 2005/06/25 09:00
PHST- 2005/06/25 09:00 [pubmed]
PHST- 2005/08/17 09:00 [medline]
PHST- 2005/06/25 09:00 [entrez]
AID - 51/7/1102 [pii]
AID - 10.1373/clinchem.2004.047084 [doi]
PST - ppublish
SO  - Clin Chem. 2005 Jul;51(7):1102-9. doi: 10.1373/clinchem.2004.047084.