PMID- 15976326
OWN - NLM
STAT- MEDLINE
DCOM- 20060106
LR  - 20061115
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Linking)
VI  - 25
IP  - 9
DP  - 2005 Sep
TI  - Mast cell tryptase in mast cell granules enhances MCP-1 and interleukin-8 
      production in human endothelial cells.
PG  - 1858-63
AB  - OBJECTIVE: Recent studies have highlighted the pathogenetic importance of chronic 
      inflammation in cardiovascular disorders such as congestive heart failure and 
      atherosclerosis. Mast cells release a wide variety of immune mediators that may 
      initiate inflammatory responses, whereas endothelial cells (ECs) play a prominent 
      role in the pathogenesis of cardiovascular diseases by secreting cytokines. The 
      purpose of this study was to clarify the role of mast cells as an activator of 
      ECs. METHODS AND RESULTS: ECs harvested from human umbilical cord veins were 
      stimulated with mast cell granules (MCGs) prepared from sonicated human leukemic 
      mast cells. The supernatants and total RNA from cells were collected. Levels of 
      interleukin (IL)-1beta, tumor necrosis factor-alpha, and granulocyte 
      colony-stimulating factor remained unchanged up to 24 hours. In contrast, levels 
      of monocyte chemoattractant protein-1 (MCP-1) and IL-8 increased significantly 
      within 6 hours. Northern blot analysis revealed an increase in MCP-1 and IL-8 
      mRNA expression in MCG-treated ECs. Induction of these chemokines was attenuated 
      by antitryptase neutralizing antibody. Furthermore, MCP-1 and IL-8 were induced 
      in ECs by incubation with human mast cell tryptase, but not with chymase. 
      CONCLUSIONS: These results indicate that the production of MCP-1 and IL-8 in ECs 
      was induced by MCG and amplified by tryptase.
FAU - Kinoshita, Makoto
AU  - Kinoshita M
AD  - Department of Cardiovascular Medicine, Kyoto University Graduate School of 
      Medicine, 54 Kawaharacho Shogoin, Kyoto, 606-8397, Japan.
FAU - Okada, Masaharu
AU  - Okada M
FAU - Hara, Masatake
AU  - Hara M
FAU - Furukawa, Yutaka
AU  - Furukawa Y
FAU - Matsumori, Akira
AU  - Matsumori A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050623
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.39 (Chymases)
RN  - EC 3.4.21.59 (Tryptases)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Chymases
MH  - Cytoplasmic Granules/enzymology
MH  - Endothelium, Vascular/cytology/immunology/*metabolism
MH  - Gene Expression
MH  - Humans
MH  - Interleukin-8/genetics/*metabolism
MH  - Mast Cells/*enzymology
MH  - Serine Endopeptidases/*metabolism
MH  - Tryptases
MH  - Umbilical Veins/cytology
MH  - Vasculitis/immunology/*metabolism
EDAT- 2005/06/25 09:00
MHDA- 2006/01/07 09:00
CRDT- 2005/06/25 09:00
PHST- 2005/06/25 09:00 [pubmed]
PHST- 2006/01/07 09:00 [medline]
PHST- 2005/06/25 09:00 [entrez]
AID - 01.ATV.0000174797.71708.97 [pii]
AID - 10.1161/01.ATV.0000174797.71708.97 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2005 Sep;25(9):1858-63. doi: 
      10.1161/01.ATV.0000174797.71708.97. Epub 2005 Jun 23.