PMID- 15978283
OWN - NLM
STAT- MEDLINE
DCOM- 20051027
LR  - 20091119
IS  - 0145-305X (Print)
IS  - 0145-305X (Linking)
VI  - 29
IP  - 10
DP  - 2005
TI  - A novel soluble form of the CSF-1 receptor inhibits proliferation of 
      self-renewing macrophages of goldfish (Carassius auratus L.).
PG  - 879-94
AB  - Macrophage colony-stimulating factor (CSF-1) is the principal regulator of the 
      survival, proliferation, and differentiation of macrophages and their precursors. 
      CSF-1 activity is tightly controlled through mechanisms regulating gene 
      expression of CSF-1 and its membrane-bound receptor (CSF-1R), as well as by 
      receptor-mediated endocytosis, metabolic processing, and inhibition of downstream 
      signaling. Herein we describe a novel mechanism for control of CSF-1 activity. 
      Spontaneously growing goldfish (Carassius auratus L.) macrophages actively 
      produced a soluble form of CSF-1R (sCSF-1R) that appears to compete for ligand 
      binding with membrane CSF-1R. The sCSF-1R transcript encodes only for the 
      ligand-binding portion of the receptor, but is derived from a full-length mRNA 
      species as determined by sequence analysis. Gene expression was associated with 
      decreased proliferation and differentiation of primary macrophages, decreased 
      growth factor activity in culture supernatants, and marked phenotypic changes 
      that culminated in apoptotic cell death. Recombinant sCSF-1R inhibited macrophage 
      proliferation at nanomolar concentrations. Antibodies against recombinant sCSF-1R 
      identified native sCSF-1R in primary macrophage culture supernatants and fish 
      serum, and suggested the presence of endogenous mechanisms temporally regulating 
      sCSF-1R release. This is the first report of a soluble CSF-1R and points to 
      intrinsic mechanisms of hematopoietic control that may be conserved across 
      evolution in discrete self-renewing macrophage populations.
FAU - Barreda, Daniel R
AU  - Barreda DR
AD  - Department of Biological Science, University of Alberta, Edmonton, AB T6G 2E9, 
      Canada.
FAU - Hanington, Patrick C
AU  - Hanington PC
FAU - Stafford, James L
AU  - Stafford JL
FAU - Belosevic, Miodrag
AU  - Belosevic M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050407
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Gene Expression
MH  - Goldfish/genetics/*immunology
MH  - Kidney/cytology/immunology
MH  - Macrophages/classification/cytology/*immunology
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics
MH  - Receptor, Macrophage Colony-Stimulating Factor/genetics/*immunology
MH  - Recombinant Proteins/genetics/immunology
MH  - Sequence Homology, Amino Acid
MH  - Solubility
EDAT- 2005/06/28 09:00
MHDA- 2005/10/28 09:00
CRDT- 2005/06/28 09:00
PHST- 2004/11/10 00:00 [received]
PHST- 2005/02/10 00:00 [accepted]
PHST- 2005/06/28 09:00 [pubmed]
PHST- 2005/10/28 09:00 [medline]
PHST- 2005/06/28 09:00 [entrez]
AID - S0145-305X(05)00025-X [pii]
AID - 10.1016/j.dci.2005.02.006 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2005;29(10):879-94. doi: 10.1016/j.dci.2005.02.006. Epub 2005 
      Apr 7.