PMID- 15979209
OWN - NLM
STAT- MEDLINE
DCOM- 20050908
LR  - 20221207
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 47
IP  - 5
DP  - 2005 Oct
TI  - Differences in the in vivo dynamics of neurotransmitter release and serotonin 
      uptake after acute para-methoxyamphetamine and 3,4-methylenedioxymethamphetamine 
      revealed by chronoamperometry.
PG  - 350-61
AB  - Illicit use of p-methoxyamphetamine (PMA) is rapidly increasing. However, little 
      is known about the acute effects of PMA on neurotransmission in vivo. High-speed 
      chronoamperometry was used to monitor neurotransmitter release and clearance in 
      anesthetized rats after local application of PMA or 
      3,4-methylenedioxymethamphetamine (MDMA). In striatum, PMA caused less 
      neurotransmitter release than MDMA. PMA-evoked release could be partially blocked 
      by pre-treatment with a serotonin (5-HT) reuptake inhibitor, suggesting that 
      evoked 5-HT release contributed to the electrochemical signal and was mediated by 
      the 5-HT transporter (SERT). MDMA-evoked release was not blocked by a SERT 
      inhibitor, suggesting that primarily DA was released. To study the effect of 
      these amphetamines on clearance of 5-HT mediated specifically by the SERT, 
      clearance of exogenously applied 5-HT was measured in the CA3 region of the 
      hippocampus. In contrast to the striatum where 5-HT is cleared by both the SERT 
      and the dopamine transporter (DAT), 5-HT is cleared primarily by the SERT in the 
      CA3 region. This is also a region where neither PMA nor MDMA evoked release of 
      neurotransmitter. The maximal inhibition of 5-HT clearance was greater after PMA 
      than MDMA. These data demonstrate in vivo (1) brain region variability in the 
      ability of PMA and MDMA to evoke release of neurotransmitter; (2) that clearance 
      of 5-HT in the striatum is mediated by both the SERT and the DAT; (3) distinct 
      differences in the amount and nature of neurotransmitter released in the striatum 
      after local application of PMA and MDMA and (4) that PMA is a more efficacious 
      inhibitor of 5-HT clearance in the hippocampus than MDMA. These fundamental 
      differences may account for the more severe adverse reactions seen clinically 
      after PMA, compared to MDMA.
FAU - Callaghan, Paul D
AU  - Callaghan PD
AD  - Department of Physiology, The University of Texas Health Science Center at San 
      Antonio, San Antonio, 78229-3900, USA.
FAU - Irvine, Rodney J
AU  - Irvine RJ
FAU - Daws, Lynette C
AU  - Daws LC
LA  - eng
GR  - MH64489/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Amphetamines)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (Piperazines)
RN  - 0 (Serotonin Agents)
RN  - 0 (Serotonin Uptake Inhibitors)
RN  - 333DO1RDJY (Serotonin)
RN  - 3J928617DW (Zimeldine)
RN  - 90X28IKH43 (vanoxerine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - OVB8F8P39Q (4-methoxyamphetamine)
SB  - IM
MH  - Amphetamines/*pharmacology
MH  - Animals
MH  - Calibration
MH  - Dose-Response Relationship, Drug
MH  - Electrochemistry
MH  - Electrodes
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Neurotransmitter Agents/*metabolism
MH  - Oxidation-Reduction
MH  - Piperazines/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serotonin/*metabolism
MH  - Serotonin Agents/*pharmacology
MH  - Selective Serotonin Reuptake Inhibitors/pharmacology
MH  - Zimeldine/pharmacology
EDAT- 2005/06/28 09:00
MHDA- 2005/09/09 09:00
CRDT- 2005/06/28 09:00
PHST- 2004/09/09 00:00 [received]
PHST- 2005/01/20 00:00 [revised]
PHST- 2005/04/06 00:00 [accepted]
PHST- 2005/06/28 09:00 [pubmed]
PHST- 2005/09/09 09:00 [medline]
PHST- 2005/06/28 09:00 [entrez]
AID - S0197-0186(05)00118-X [pii]
AID - 10.1016/j.neuint.2005.04.026 [doi]
PST - ppublish
SO  - Neurochem Int. 2005 Oct;47(5):350-61. doi: 10.1016/j.neuint.2005.04.026.