PMID- 15979299
OWN - NLM
STAT- MEDLINE
DCOM- 20060317
LR  - 20191210
IS  - 0956-5663 (Print)
IS  - 0956-5663 (Linking)
VI  - 21
IP  - 7
DP  - 2006 Jan 15
TI  - Molecularly imprinted thin film self-assembled on piezoelectric quartz crystal 
      surface by the sol-gel process for protein recognition.
PG  - 1244-51
AB  - A novel method of combining sol-gel and self-assembly technology to prepare a 
      human serum albumin (HSA)-imprinted film on the surface of piezoelectric quartz 
      crystal (PQC) Au-electrode modified with thioglycolic acid was described in this 
      paper. The imprinting process was characterized by using the piezoelectric quartz 
      crystal impedance (PQCI) technique and electrochemical impedance technique. 
      Scanning electron microscope (SEM) was employed to characterize the surface 
      morphology of the resultant imprinted film. The piezoelectric technique and 
      electrochemical impedance technique were also employed to investigate the binding 
      performance of the sol-gel-imprinted film with the template protein. The results 
      showed that the imprinted PQC film can give selective recognition to the template 
      protein. The effects of salts and solvents on the binding capacity of the 
      imprinted film with protein were discussed in detail. Other influencing factors 
      (temperature and pH) have also been investigated. This self-assembly sol-gel 
      imprinting technique was proved to be an alternative method for the preparation 
      of biomacromolecule-imprinted thin film.
FAU - Zhang, Zhaohui
AU  - Zhang Z
AD  - State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry 
      and Chemical Engineering, Hunan University, Changsha 410082, China.
FAU - Long, Yumei
AU  - Long Y
FAU - Nie, Lihua
AU  - Nie L
FAU - Yao, Shouzhuo
AU  - Yao S
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050623
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Membranes, Artificial)
RN  - 0 (Serum Albumin)
RN  - 14808-60-7 (Quartz)
SB  - IM
MH  - Adsorption
MH  - Biosensing Techniques/*methods
MH  - Crystallization/*methods
MH  - Electrochemistry/*instrumentation/methods
MH  - Feasibility Studies
MH  - Humans
MH  - *Membranes, Artificial
MH  - Phase Transition
MH  - Protein Array Analysis/*methods
MH  - Protein Binding
MH  - Quartz
MH  - Serum Albumin/*analysis/*chemistry
MH  - Surface Properties
MH  - *Transducers
EDAT- 2005/06/28 09:00
MHDA- 2006/03/18 09:00
CRDT- 2005/06/28 09:00
PHST- 2005/03/30 00:00 [received]
PHST- 2005/05/19 00:00 [revised]
PHST- 2005/05/23 00:00 [accepted]
PHST- 2005/06/28 09:00 [pubmed]
PHST- 2006/03/18 09:00 [medline]
PHST- 2005/06/28 09:00 [entrez]
AID - S0956-5663(05)00144-2 [pii]
AID - 10.1016/j.bios.2005.05.009 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2006 Jan 15;21(7):1244-51. doi: 10.1016/j.bios.2005.05.009. 
      Epub 2005 Jun 23.