PMID- 15981811
OWN - NLM
STAT- MEDLINE
DCOM- 20050719
LR  - 20131121
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 123
IP  - 5
DP  - 2005 May
TI  - High-throughput cervical cancer screening using intracellular human 
      papillomavirus E6 and E7 mRNA quantification by flow cytometry.
PG  - 716-23
AB  - The Papanicolaou (Pap) test, based solely on the morphologic examination of 
      exfoliated cells from the cervix, has reduced deaths due to cervical cancer by 
      74% in the United States during the past 40 years. During that time, the 
      molecular mechanisms of cervical cancer have largely been elucidated. Taken 
      together, these observations have identified a need for a high-throughput 
      cervical cancer screening assay. We report the development of a high-throughput 
      assay consisting of simultaneous immunophenotyping and ultrasensitive in situ 
      hybridization for human papillomavirus (HPV) E6 and E7 messenger RNA (mRNA). This 
      assay can be performed in less than 3 hours directly from liquid-based cervical 
      cytology specimens. Overall, HPV fluorescence in situ hybridization (FISH) for E6 
      and E7 mRNA demonstrated 83.3% sensitivity and 91.3% specificity for high-grade 
      squamous intraepithelial lesions compared with the Pap test in 231 liquid-based 
      cytology samples from 2 cohorts. In a subset of these samples, HPV FISH 
      demonstrated higher sensitivity and specificity than Hybrid Capture (Digene, 
      Gaithersburg, MD) for high-risk genotypes.
FAU - Narimatsu, Roberto
AU  - Narimatsu R
AD  - Department of Pathology and Medicine, Division of Infectious Diseases and 
      Geographic Medicine, Stanford University Medical School, CA 94305-5629, USA.
FAU - Patterson, Bruce K
AU  - Patterson BK
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (E6 protein, Human papillomavirus type 16)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Papillomavirus E7 Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Repressor Proteins)
RN  - 0 (oncogene protein E7, Human papillomavirus type 16)
SB  - IM
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Mass Screening/*methods
MH  - Oncogene Proteins, Viral/*genetics/metabolism
MH  - Papanicolaou Test
MH  - Papillomaviridae/genetics/*isolation & purification
MH  - Papillomavirus E7 Proteins
MH  - Papillomavirus Infections/*diagnosis
MH  - RNA, Messenger/analysis
MH  - RNA, Viral/isolation & purification/*metabolism
MH  - Repressor Proteins/genetics/metabolism
MH  - Sensitivity and Specificity
MH  - *Uterine Cervical Neoplasms/diagnosis/metabolism/virology
MH  - Vaginal Smears/instrumentation/methods
EDAT- 2005/06/29 09:00
MHDA- 2005/07/20 09:00
CRDT- 2005/06/29 09:00
PHST- 2005/06/29 09:00 [pubmed]
PHST- 2005/07/20 09:00 [medline]
PHST- 2005/06/29 09:00 [entrez]
PST - ppublish
SO  - Am J Clin Pathol. 2005 May;123(5):716-23.