PMID- 15981815
OWN - NLM
STAT- MEDLINE
DCOM- 20050719
LR  - 20061115
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 123
IP  - 5
DP  - 2005 May
TI  - Multitarget FISH and LOH analyses at chromosome 3p in non-small cell lung cancer 
      and adjacent bronchial epithelium.
PG  - 752-61
AB  - Multitarget fluorescence in situ hybridization (FISH; LAVysion, Vysis, Downers 
      Grove, IL) targeting chromosomes 6p11-q11, 7p12, 8q24, and 5p15.2 was compared 
      with results of microsatellite studies at chromosome 3p to identify molecular 
      changes associated with tobacco use and tumor development in non-small cell lung 
      cancer (NSCLC). Analyses were performed on 26 NSCLCs and matched benign bronchial 
      epithelium; samples from 10 patients without NSCLC served as control samples. 
      Significant molecular differences between tumor tissue and corresponding benign 
      bronchi were found using FISH (P = .001) and loss of heterozygosity (LOH) 
      analysis (P = .031). Bronchial epithelium from patients with NSCLC was 
      genetically different from epithelium from patients without NSCLC in FISH 
      analysis (P = .025). Receiver operating characteristic curve analysis revealed an 
      optimal cutoff value of 5% atypical cells for bronchial epithelium. There was no 
      statistical correlation with the patient's smoking history, and LOH analysis of 
      bronchi did not differentiate between patients with and without NSCLC. Multicolor 
      FISH analysis is able to detect a tumor-associated molecular field effect in 
      bronchi adjacent to NSCLC.
FAU - Woenckhaus, Matthias
AU  - Woenckhaus M
AD  - Department of Pathology, University of Regensburg, Germany.
FAU - Grepmeier, Ulrike
AU  - Grepmeier U
FAU - Wild, Peter J
AU  - Wild PJ
FAU - Merk, Johannes
AU  - Merk J
FAU - Pfeifer, Michael
AU  - Pfeifer M
FAU - Woenckhaus, Ulrike
AU  - Woenckhaus U
FAU - Stoelcker, Benjamin
AU  - Stoelcker B
FAU - Blaszyk, Hagen
AU  - Blaszyk H
FAU - Hofstaedter, Ferdinand
AU  - Hofstaedter F
FAU - Dietmaier, Wolfgang
AU  - Dietmaier W
FAU - Hartmann, Arndt
AU  - Hartmann A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bronchi/*pathology
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/pathology
MH  - Cell Count
MH  - *Chromosomes, Human, Pair 3
MH  - DNA, Neoplasm/analysis
MH  - Epithelium/pathology
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Loss of Heterozygosity
MH  - Lung Neoplasms/*genetics/pathology
MH  - Male
MH  - Microsatellite Repeats
MH  - Middle Aged
MH  - ROC Curve
EDAT- 2005/06/29 09:00
MHDA- 2005/07/20 09:00
CRDT- 2005/06/29 09:00
PHST- 2005/06/29 09:00 [pubmed]
PHST- 2005/07/20 09:00 [medline]
PHST- 2005/06/29 09:00 [entrez]
PST - ppublish
SO  - Am J Clin Pathol. 2005 May;123(5):752-61.