PMID- 15982342
OWN - NLM
STAT- MEDLINE
DCOM- 20050930
LR  - 20191210
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 130
IP  - 1
DP  - 2005 Jul
TI  - Interphase fluorescence in situ hybridization with an IGH probe is important in 
      the evaluation of patients with a clinical diagnosis of chronic lymphocytic 
      leukaemia.
PG  - 36-42
AB  - Translocations involving IGH are common in some lymphoid malignancies but are 
      believed to be rare in chronic lymphocytic leukaemia (CLL). To study the clinical 
      utility of fluorescence in situ hybridization (FISH) for IGH translocations, we 
      reviewed 1032 patients with a presumptive diagnosis of CLL. Seventy-six (7%) 
      patients had IGH translocations. Pathology and clinical data were available for 
      the 24 patients evaluated at the Mayo Clinic. Ten (42%) patients had IGH/cyclin 
      D1 fusion and were diagnosed with mantle cell lymphoma (MCL). The immunophenotype 
      was typical of MCL in three of these patients and atypical for MCL in seven 
      patients. One patient had biclonal disease with typical MCL and CLL with 
      IGH/BCL-2. Eleven (46%) patients had IGH/BCL-2 fusion including the patient with 
      biclonal disease. Two of these patients had leukaemic phase follicular lymphoma 
      and nine patients had CLL. The median progression-free survival of patients with 
      CLL and IGH/BCL-2 translocation was 20.6 months. The two patients with IGH/BCL-3 
      fusion (one of these also had IGH/BCL-11a) had rapid disease progression. The IGH 
      partner gene was not identified in two patients. We conclude that use of an IGH 
      probe in FISH analysis of monoclonal B-cell lymphocytosis improves diagnostic 
      precision and could have prognostic value in patients with CLL.
FAU - Nowakowski, G S
AU  - Nowakowski GS
AD  - Division of Hematology, Mayo Clinic College of Medicine, Rochester, MN 55905, 
      USA.
FAU - Dewald, G W
AU  - Dewald GW
FAU - Hoyer, J D
AU  - Hoyer JD
FAU - Paternoster, S F
AU  - Paternoster SF
FAU - Stockero, K J
AU  - Stockero KJ
FAU - Fink, S R
AU  - Fink SR
FAU - Smoley, S A
AU  - Smoley SA
FAU - Remstein, E D
AU  - Remstein ED
FAU - Phyliky, R L
AU  - Phyliky RL
FAU - Call, T G
AU  - Call TG
FAU - Shanafelt, T D
AU  - Shanafelt TD
FAU - Kay, N E
AU  - Kay NE
FAU - Zent, C S
AU  - Zent CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (B-Cell Lymphoma 3 Protein)
RN  - 0 (BCL3 protein, human)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Transcription Factors)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - B-Cell Lymphoma 3 Protein
MH  - Cyclin D1/genetics
MH  - Diagnosis, Differential
MH  - Flow Cytometry
MH  - *Genes, Immunoglobulin
MH  - Genes, bcl-2
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*diagnosis/genetics/immunology
MH  - Lymphoma, B-Cell/diagnosis/genetics
MH  - Lymphoma, Mantle-Cell/diagnosis/genetics
MH  - *Oligonucleotide Probes
MH  - Proto-Oncogene Proteins/genetics
MH  - Transcription Factors
MH  - *Translocation, Genetic
EDAT- 2005/06/29 09:00
MHDA- 2005/10/01 09:00
CRDT- 2005/06/29 09:00
PHST- 2005/06/29 09:00 [pubmed]
PHST- 2005/10/01 09:00 [medline]
PHST- 2005/06/29 09:00 [entrez]
AID - BJH5548 [pii]
AID - 10.1111/j.1365-2141.2005.05548.x [doi]
PST - ppublish
SO  - Br J Haematol. 2005 Jul;130(1):36-42. doi: 10.1111/j.1365-2141.2005.05548.x.