PMID- 15982568
OWN - NLM
STAT- MEDLINE
DCOM- 20050922
LR  - 20141120
IS  - 0966-3274 (Print)
IS  - 0966-3274 (Linking)
VI  - 14
IP  - 3-4
DP  - 2005 Aug
TI  - In vitro preparation and functional assessment of human monocyte-derived 
      dendritic cells-potential antigen-specific modulators of in vivo immune 
      responses.
PG  - 231-44
AB  - Dendritic cells (DCs) are highly specialized professional antigen presenting 
      cells that have a potent capacity for stimulating naive, memory and effector 
      T-cells. They are located in lymphoid organs as well as in almost all nonlymphoid 
      tissues. Immature DCs, residing in the host microenvironment, respond to danger 
      signals with maturation, a differentiation process along which they acquire the 
      ability to direct the extent and the type of primary immune responses according 
      to the type of danger perceived. In this review we present some of our approaches 
      and experiences regarding the isolation of human monocytes from peripheral blood 
      and the in vitro preparation of, first, immature and then mature DCs by applying 
      several maturation factors: bacterial lipopolysaccharide (LPS), a defined mixture 
      of recombinant pro-inflammatory cytokines, monocyte conditioned medium (MCM) and 
      TNF-alpha alone. The assessment of DC phenotypes and their functional 
      capabilities as well as some of the techniques used for tumour associated antigen 
      loading are also presented. The results of such studies represent a basis for 
      optimal in vitro preparation of DCs, which could be clinically used to modulate 
      immune responses in cancer, autoimmune diseases and in the planned onset of 
      tolerance to disparate major histocompatibilty complex (MHC) antigens prior to 
      tissue or organ transplantation.
FAU - Jeras, Matjaz
AU  - Jeras M
AD  - Blood Transfusion Centre of Slovenia, Tissue Typing Center, Slajmerjeva 6, 
      SI-1000 Ljubljana, Slovenia. matjaz.jeras@ztm.si
FAU - Bergant, Martina
AU  - Bergant M
FAU - Repnik, Urska
AU  - Repnik U
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20050426
PL  - Netherlands
TA  - Transpl Immunol
JT  - Transplant immunology
JID - 9309923
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
SB  - IM
MH  - Antigen Presentation/immunology
MH  - Cell Culture Techniques
MH  - Cell Differentiation/*immunology
MH  - Cell Lineage
MH  - Cell Movement/immunology
MH  - Culture Media, Conditioned
MH  - Cytokines
MH  - Dendritic Cells/*cytology/*immunology
MH  - Genetic Therapy/methods
MH  - Humans
MH  - Monocytes/*cytology/immunology
MH  - Transfection
RF  - 77
EDAT- 2005/06/29 09:00
MHDA- 2005/09/24 09:00
CRDT- 2005/06/29 09:00
PHST- 2005/03/14 00:00 [accepted]
PHST- 2005/06/29 09:00 [pubmed]
PHST- 2005/09/24 09:00 [medline]
PHST- 2005/06/29 09:00 [entrez]
AID - S0966-3274(05)00041-9 [pii]
AID - 10.1016/j.trim.2005.03.012 [doi]
PST - ppublish
SO  - Transpl Immunol. 2005 Aug;14(3-4):231-44. doi: 10.1016/j.trim.2005.03.012. Epub 
      2005 Apr 26.