PMID- 15983403
OWN - NLM
STAT- MEDLINE
DCOM- 20050914
LR  - 20190523
IS  - 0950-1991 (Print)
IS  - 0950-1991 (Linking)
VI  - 132
IP  - 14
DP  - 2005 Jul
TI  - Zebrafish penner/lethal giant larvae 2 functions in hemidesmosome formation, 
      maintenance of cellular morphology and growth regulation in the developing basal 
      epidermis.
PG  - 3255-65
AB  - Epithelial cells are equipped with junctional complexes that are involved in 
      maintaining tissue architecture, providing mechanical integrity and suppressing 
      tumour formation as well as invasiveness. A strict spatial segregation of these 
      junctional complexes leads to the polarisation of epithelial cells. In vertebrate 
      epithelia, basally localised hemidesmosomes mediate stable adhesion between 
      epithelial cells and the underlying basement membrane. Although components of 
      hemidesmosomes are relatively well known, the molecular machinery involved in 
      governing the formation of these robust junctions, remains elusive. Here, we have 
      identified the first component of this machinery using a forward genetic approach 
      in zebrafish as we show that the function of penner (pen)/lethal giant larvae 2 
      (lgl2) is necessary for hemidesmosome formation and maintenance of the tissue 
      integrity in the developing basal epidermis. Moreover, in pen/lgl2 mutant, basal 
      epidermal cells hyper-proliferate and migrate to ectopic positions. Of the two 
      vertebrate orthologues of the Drosophila tumour suppressor gene lethal giant 
      larvae, the function of lgl2 in vertebrate development and organogenesis remained 
      unclear so far. Here, we have unravelled an essential function of lgl2 during 
      development of the epidermis in vertebrates.
FAU - Sonawane, Mahendra
AU  - Sonawane M
AD  - Max-Planck Institut fur Entwicklungsbiologie, Department of Genetics, 
      Spemannstrasse 35, Tuebingen, D-72076, Germany. 
      mahendra.sonawane@tuebingen.mpg.de
FAU - Carpio, Yamila
AU  - Carpio Y
FAU - Geisler, Robert
AU  - Geisler R
FAU - Schwarz, Heinz
AU  - Schwarz H
FAU - Maischein, Hans-Martin
AU  - Maischein HM
FAU - Nuesslein-Volhard, Christiane
AU  - Nuesslein-Volhard C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Development
JT  - Development (Cambridge, England)
JID - 8701744
RN  - 0 (Drosophila Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (l(2)gl protein, Drosophila)
RN  - 0 (llgl2 protein, zebrafish)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Drosophila Proteins/genetics/*physiology
MH  - *Epidermal Cells
MH  - Epidermis/*embryology/growth & development
MH  - *Genes, Lethal
MH  - Hemidesmosomes/*metabolism
MH  - Microscopy, Interference
MH  - Molecular Sequence Data
MH  - Tumor Suppressor Proteins/genetics/*physiology
MH  - Zebrafish/*embryology
MH  - Zebrafish Proteins/genetics/*metabolism
EDAT- 2005/06/29 09:00
MHDA- 2005/09/15 09:00
CRDT- 2005/06/29 09:00
PHST- 2005/06/29 09:00 [pubmed]
PHST- 2005/09/15 09:00 [medline]
PHST- 2005/06/29 09:00 [entrez]
AID - 132/14/3255 [pii]
AID - 10.1242/dev.01904 [doi]
PST - ppublish
SO  - Development. 2005 Jul;132(14):3255-65. doi: 10.1242/dev.01904.