PMID- 15983676
OWN - NLM
STAT- MEDLINE
DCOM- 20071002
LR  - 20080623
IS  - 1658-3175 (Electronic)
IS  - 0379-5284 (Linking)
VI  - 26
IP  - 6
DP  - 2005 Jun
TI  - Comparative study on apoptosis in the testes of normal and alcoholic rats.
PG  - 928-33
AB  - OBJECTIVES: To investigate the tissue injury associated with long-term alcohol 
      consumption in male gonads. To this end, apoptotic testicle tissues of alcoholic 
      rats were compared with the testicle tissues of the control rats. METHODS: This 
      study was conducted in the Department of Anatomy and Division of Pathology, 
      Faculty of Medicine, Kocaeli University, Kocaeli, Turkey during the period 2002 
      to 2003. We used Sprague-Dawley rats as the subject material in the investigation 
      of apoptosis. We divided the rats into 2 groups: alcoholic rats and the control 
      group, with 10 adult male rats in each group. We housed the subjects in each 
      group under controlled temperature (22 +/- 3 degrees C) and humidity (62 +/- 7%) 
      and lighting (12 hours darkness and 12 hours daylight per day). We fed the rats 
      in the alcoholic group by ethanol in liquid diet for 12 weeks while the control 
      rats received the normal isocaloric diet. We fixed the testicle tissues of both 
      groups by perfusion of 10% formaldehyde through left ventricle and then removed. 
      We further fixed the tissues in formaldehyde solution for at least 2 days. After 
      dehydration by ethanol, we embedded the tissue in paraffin and used serial 
      paraffin sections (5 micron thickness) for immunohistochemistry. We used 
      Caspase-3 Ab-4 (CPP32) antibody to identify caspase reaction in apoptotic 
      regions. Hence, we observed the stained sections and photographed the apoptotic 
      seminiferous tubules (ST). For comparison of apoptosis in the alcoholic group and 
      control group, we counted the apoptotic germ cells in x 400 magnification under 
      light microscope. RESULTS: The diameters of seminiferous tubules were measured 
      using light microscope with micrometre. The findings were compared by computer 
      and significant differences in apoptosis between 2 groups (p<0.01) were detected. 
      CONCLUSION: Apoptosis is significantly induced in ethanol treated rat related to 
      the overuse of ethanol. The findings indicate the tissue injury of testicles 
      associated with alcohol consumption.
FAU - Bamac, Yalin
AU  - Bamac Y
AD  - Department of Anatomy, School of Medicine, Kocaeli University, Kocaeli, Turkey.
FAU - Colak, Tuncay
AU  - Colak T
FAU - Bamac, Belgin
AU  - Bamac B
FAU - Ercin, Cengiz
AU  - Ercin C
FAU - Ozturk, Baris
AU  - Ozturk B
FAU - Ozbek, Aydin
AU  - Ozbek A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Saudi Arabia
TA  - Saudi Med J
JT  - Saudi medical journal
JID - 7909441
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Alcoholism/metabolism/*pathology
MH  - Animals
MH  - *Apoptosis
MH  - Caspases/metabolism
MH  - Germ Cells/pathology
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seminiferous Tubules/pathology
MH  - Testis/metabolism/*pathology
EDAT- 2005/06/29 09:00
MHDA- 2007/10/03 09:00
CRDT- 2005/06/29 09:00
PHST- 2005/06/29 09:00 [pubmed]
PHST- 2007/10/03 09:00 [medline]
PHST- 2005/06/29 09:00 [entrez]
AID - 20040922' [pii]
PST - ppublish
SO  - Saudi Med J. 2005 Jun;26(6):928-33.