PMID- 15986888
OWN - NLM
STAT- MEDLINE
DCOM- 20050930
LR  - 20191109
IS  - 1006-9305 (Print)
IS  - 1006-9305 (Linking)
VI  - 48
IP  - 2
DP  - 2005 Apr
TI  - Monitoring of microbial community structure and succession in the biohydrogen 
      production reactor by denaturing gradient gel electrophoresis (DGGE).
PG  - 155-62
AB  - To study the structure of microbial communities in the biological hydrogen 
      production reactor and determine the ecological function of hydrogen producing 
      bacteria, anaerobic sludge was obtained from the continuous stirred tank reactor 
      (CSTR) in different periods of time, and the diversity and dynamics of microbial 
      communities were investigated by denaturing gradient gel electrophoresis (DGGE). 
      The results of DGGE demonstrated that an obvious shift of microbial population 
      happened from the beginning of star-up to the 28th day, and the ethanol type 
      fermentation was established. After 28 days the structure of microbial community 
      became stable, and the climax community was formed. Comparative analysis of 16S 
      rDNA sequences from reamplifying and sequencing the prominent bands indicated 
      that the dominant population belonged to low G+C Gram-positive bacteria 
      (Clostridium sp. and Ethanologenbacterium sp.), beta-proteobacteria (Acidovorax 
      sp.), gamma-proteobacteria (Kluyvera sp.), Bacteroides (uncultured bacterium 
      SJA-168), and Spirochaetes (uncultured eubacterium E1-K13), respectively. The 
      hydrogen production rate increased obviously with the increase of 
      Ethanologenbacterium sp., Clostridium sp. and uncultured Spirochaetes after 21 
      days, meanwhile the succession of ethanol type fermentation was formed. 
      Throughout the succession the microbial diversity increased however it decreased 
      after 21 days. Some types of Clostridium sp. Acidovorax sp., Kluyvera sp., and 
      Bacteroides were dominant populations during all periods of time. These special 
      populations were essential for the construction of climax community. Hydrogen 
      production efficiency was dependent on both hydrogen producing bacteria and other 
      populations. It implied that the co-metabolism of microbial community played a 
      great role of biohydrogen production in the reactors.
FAU - Xing, Defeng
AU  - Xing D
AD  - School of Municipal and Environmental Engineering, Harbin Institute of 
      Technology, Harbin 150090, China.
FAU - Ren, Nanqi
AU  - Ren N
FAU - Gong, Manli
AU  - Gong M
FAU - Li, Jianzheng
AU  - Li J
FAU - Li, Qiubo
AU  - Li Q
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sci China C Life Sci
JT  - Science in China. Series C, Life sciences
JID - 9611809
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 3K9958V90M (Ethanol)
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Bacteroides/genetics/metabolism
MH  - Base Sequence
MH  - *Bioreactors
MH  - Chromatography, Gas
MH  - *Electrophoresis, Gel, Pulsed-Field
MH  - Ethanol/metabolism
MH  - Fermentation
MH  - Gram-Positive Bacteria/genetics/metabolism
MH  - Hydrogen/*metabolism
MH  - Phylogeny
MH  - Proteobacteria/genetics/metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/microbiology
MH  - Spirochaetales/genetics/metabolism
MH  - Time Factors
MH  - Waste Disposal, Fluid
EDAT- 2005/07/01 09:00
MHDA- 2005/10/01 09:00
CRDT- 2005/07/01 09:00
PHST- 2005/07/01 09:00 [pubmed]
PHST- 2005/10/01 09:00 [medline]
PHST- 2005/07/01 09:00 [entrez]
AID - 10.1007/BF02879668 [doi]
PST - ppublish
SO  - Sci China C Life Sci. 2005 Apr;48(2):155-62. doi: 10.1007/BF02879668.