PMID- 15987099
OWN - NLM
STAT- MEDLINE
DCOM- 20070316
LR  - 20081121
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 77
IP  - 13
DP  - 2005 Jul 1
TI  - Stable permanently hydrophilic protein-resistant thin-film coatings on 
      poly(dimethylsiloxane) substrates by electrostatic self-assembly and chemical 
      cross-linking.
PG  - 3971-8
AB  - Poly(dimethylsiloxane) (PDMS) is a biomaterial that presents serious surface 
      instability characterized by hydrophobicity recovery. Permanently hydrophilic 
      PDMS surfaces were created using electrostatic self-assembly of polyethyleneimine 
      and poly(acrylic acid) on top of a hydrolyzed poly(styrene-alt-maleic anhydride) 
      base layer adsorbed on PDMS. Cross-linking of the polyelectrolyte multilayers 
      (PEMS) by carbodiimide coupling and covalent attachment of poly(ethylene glycol) 
      (PEG) chains to the PEMS produced stable, hydrophilic, protein-resistant 
      coatings, which resisted hydrophobicity recovery in air. Attenuated total 
      reflection Fourier transform infrared spectroscopy and X-ray photoelectron 
      spectroscopy revealed that the thin films had excellent chemical stability and 
      resisted hydrophobicity recovery in air over 77 days of measurement. The spectra 
      also showed a dense coverage for PEG dialdehyde and excellent resistance to 
      protein adsorption from undiluted rat serum. Atomic force microscopy revealed 
      dense coverage with PEG dialdehyde and PEG diamine. Contact angle measurements 
      showed that all films were hydrophilic and that the PEG dialdehyde-topped thin 
      film had a virtually constant contact angle (approximately 20 degrees ) over the 
      five months of the study. Electrokinetic analysis of the coatings in 
      microchannels always exposed to air also gave good protein separation and 
      constant electroosmotic flow during the five months that the measurements were 
      done. We expect that the stable, hydrophilic, protein-resistant thin-film 
      coatings will be useful for many applications that require long-term surface 
      stability.
FAU - Makamba, Honest
AU  - Makamba H
AD  - Department of Chemistry, National Cheng Kung University, Tainan 701, Taiwan.
FAU - Hsieh, Ya-Yu
AU  - Hsieh YY
FAU - Sung, Wang-Chou
AU  - Sung WC
FAU - Chen, Shu-Hui
AU  - Chen SH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Biocompatible Materials)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Silicones)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/*chemistry
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Plasma/*chemistry
MH  - Rats
MH  - Silicones/*chemistry
MH  - *Static Electricity
EDAT- 2005/07/01 09:00
MHDA- 2007/03/17 09:00
CRDT- 2005/07/01 09:00
PHST- 2005/07/01 09:00 [pubmed]
PHST- 2007/03/17 09:00 [medline]
PHST- 2005/07/01 09:00 [entrez]
AID - 10.1021/ac0502706 [doi]
PST - ppublish
SO  - Anal Chem. 2005 Jul 1;77(13):3971-8. doi: 10.1021/ac0502706.