PMID- 15991234
OWN - NLM
STAT- MEDLINE
DCOM- 20051014
LR  - 20161124
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 91
IP  - 3
DP  - 2005 Aug 5
TI  - Concurrent microscopic observations and activity measurements of cellulose 
      hydrolyzing and methanogenic populations during the batch anaerobic digestion of 
      crystalline cellulose.
PG  - 369-78
AB  - This study compares process data with microscopic observations from an anaerobic 
      digestion of organic particles. As the first part of the study, this article 
      presents detailed observations of microbial biofilm architecture and structure in 
      a 1.25-L batch digester where all particles are of an equal age. Microcrystalline 
      cellulose was used as the sole carbon and energy source. The digestions were 
      inoculated with either leachate from a 220-L anaerobic municipal solid waste 
      digester or strained rumen contents from a fistulated cow. The hydrolysis rate, 
      when normalized by the amount of cellulose remaining in the reactor, was found to 
      reach a constant value 1 day after inoculation with rumen fluid, and 3 days after 
      inoculating with digester leachate. A constant value of a mass specific 
      hydrolysis rate is argued to represent full colonization of the cellulose surface 
      and first-order kinetics only apply after this point. Additionally, the 
      first-order hydrolysis rate constant, once surfaces were saturated with biofilm, 
      was found to be two times higher with a rumen inoculum, compared to a digester 
      leachate inoculum. Images generated by fluorescence in situ hybridization (FISH) 
      probing and confocal laser scanning microscopy show that the microbial 
      communities involved in the anaerobic biodegradation process exist entirely 
      within the biofilm. For the reactor conditions used in these experiments, the 
      predominant methanogens exist in ball-shaped colonies within the biofilm.
CI  - Copyright 2005 Wiley Periodicals, Inc.
FAU - Song, Hyohak
AU  - Song H
AD  - School of Engineering, The University of Queensland, St. Lucia QLD4072, 
      Australia.
FAU - Clarke, William P
AU  - Clarke WP
FAU - Blackall, Linda L
AU  - Blackall LL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 9004-34-6 (Cellulose)
RN  - OP0UW79H66 (Methane)
RN  - OP1R32D61U (microcrystalline cellulose)
SB  - IM
MH  - Anaerobiosis
MH  - Animals
MH  - Bacteria, Anaerobic/cytology/genetics/*metabolism
MH  - Biodegradation, Environmental
MH  - *Biofilms
MH  - Bioreactors
MH  - Cattle
MH  - Cellulose/*metabolism/ultrastructure
MH  - Hydrolysis
MH  - In Situ Hybridization, Fluorescence
MH  - Methane/metabolism
MH  - Microscopy
MH  - Refuse Disposal/*methods
MH  - Rumen/chemistry
EDAT- 2005/07/02 09:00
MHDA- 2005/10/15 09:00
CRDT- 2005/07/02 09:00
PHST- 2005/07/02 09:00 [pubmed]
PHST- 2005/10/15 09:00 [medline]
PHST- 2005/07/02 09:00 [entrez]
AID - 10.1002/bit.20517 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2005 Aug 5;91(3):369-78. doi: 10.1002/bit.20517.