PMID- 15994791
OWN - NLM
STAT- MEDLINE
DCOM- 20050721
LR  - 20211203
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 79
IP  - 14
DP  - 2005 Jul
TI  - Human tripartite motif 5alpha domains responsible for retrovirus restriction 
      activity and specificity.
PG  - 8969-78
AB  - The tripartite motif 5alpha protein (TRIM5alpha) is one of several factors 
      expressed by mammalian cells that inhibit retrovirus replication. Human 
      TRIM5alpha (huTRIM5alpha) inhibits infection by N-tropic murine leukemia virus 
      (N-MLV) but is inactive against human immunodeficiency virus type 1 (HIV-1). 
      However, we show that replacement of a small segment in the carboxy-terminal 
      B30.2/SPRY domain of huTRIM5alpha with its rhesus macaque counterpart 
      (rhTRIM5alpha) endows it with the ability to potently inhibit HIV-1 infection. 
      The B30.2/SPRY domain and an additional domain in huTRIM5alpha, comprising the 
      amino-terminal RING and B-box components of the TRIM motif, are required for 
      N-MLV restriction activity, while the intervening coiled-coil domain is necessary 
      and sufficient for huTRIM5alpha multimerization. Truncated huTRIM5alpha proteins 
      that lack either or both the N-terminal RING/B-Box or the C-terminal B30.2/SPRY 
      domain form heteromultimers with full-length huTRIM5alpha and are dominant 
      inhibitors of its N-MLV restricting activity, suggesting that homomultimerization 
      of intact huTRIM5alpha monomers is necessary for N-MLV restriction. However, 
      localization in large cytoplasmic bodies is not required for inhibition of N-MLV 
      by huTRIM5alpha or for inhibition of HIV-1 by chimeric or rhTRIM5alpha.
FAU - Perez-Caballero, David
AU  - Perez-Caballero D
AD  - Aaron Diamond AIDS Research Center, 455 First Ave., New York, NY 10021, USA.
FAU - Hatziioannou, Theodora
AU  - Hatziioannou T
FAU - Yang, Annie
AU  - Yang A
FAU - Cowan, Simone
AU  - Cowan S
FAU - Bieniasz, Paul D
AU  - Bieniasz PD
LA  - eng
GR  - R01 AI050111/AI/NIAID NIH HHS/United States
GR  - R01 AI064003/AI/NIAID NIH HHS/United States
GR  - R01AI050111/AI/NIAID NIH HHS/United States
GR  - R01AI64003/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antiviral Agents)
RN  - 0 (Antiviral Restriction Factors)
RN  - 0 (Carrier Proteins)
RN  - 0 (Tripartite Motif Proteins)
RN  - EC 2.3.2.27 (TRIM5 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antiviral Agents/*physiology
MH  - Antiviral Restriction Factors
MH  - Carrier Proteins/*chemistry/*physiology
MH  - HIV-1/physiology
MH  - HeLa Cells
MH  - Humans
MH  - Leukemia Virus, Murine/physiology
MH  - Molecular Sequence Data
MH  - Retroviridae Infections/*prevention & control
MH  - Structure-Activity Relationship
MH  - Tripartite Motif Proteins
MH  - Ubiquitin-Protein Ligases
PMC - PMC1168745
EDAT- 2005/07/05 09:00
MHDA- 2005/07/22 09:00
PMCR- 2005/07/01
CRDT- 2005/07/05 09:00
PHST- 2005/07/05 09:00 [pubmed]
PHST- 2005/07/22 09:00 [medline]
PHST- 2005/07/05 09:00 [entrez]
PHST- 2005/07/01 00:00 [pmc-release]
AID - 79/14/8969 [pii]
AID - 0255-05 [pii]
AID - 10.1128/JVI.79.14.8969-8978.2005 [doi]
PST - ppublish
SO  - J Virol. 2005 Jul;79(14):8969-78. doi: 10.1128/JVI.79.14.8969-8978.2005.