PMID- 15995362
OWN - NLM
STAT- MEDLINE
DCOM- 20051027
LR  - 20240315
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 19
IP  - 3
DP  - 2005 Jun 30
TI  - Characterization of rDNAs and tandem repeats in the heterochromatin of Brassica 
      rapa.
PG  - 436-44
AB  - We describe the morphology and molecular organization of heterochromatin domains 
      in the interphase nuclei, and mitotic and meiotic chromosomes, of Brassica rapa, 
      using DAPI staining and fluorescence in situ hybridization (FISH) of rDNA and 
      pericentromere tandem repeats. We have developed a simple method to distinguish 
      the centromeric regions of mitotic metaphase chromosomes by prolonged irradiation 
      with UV light at the DAPI excitation wavelength. Application of this bleached 
      DAPI band (BDB) karyotyping method to the 45S and 5S rDNAs and 176 bp centromere 
      satellite repeats distinguished the 10 B. rapa chromosomes. We further 
      characterized the centromeric repeat sequences in BAC end sequences. These fell 
      into two classes, CentBr1 and CentBr2, occupying the centromeres of eight and two 
      chromosomes, respectively. The centromere satellites encompassed about 30% of the 
      total chromosomes, particularly in the core centromere blocks of all the 
      chromosomes. Interestingly, centromere length was inversely correlated with 
      chromosome length. The morphology and molecular organization of heterochromatin 
      domains in interphase nuclei, and in mitotic and meiotic chromosomes, were 
      further characterized by DAPI staining and FISH of rDNA and CentBr. The DAPI 
      fluorescence of interphase nuclei revealed ten to twenty conspicuous 
      chromocenters, each composed of the heterochromatin of up to four chromosomes 
      and/or nucleolar organizing regions.
FAU - Lim, Ki-Byung
AU  - Lim KB
AD  - Brassica Genomics Team, National Institute of Agricultural Biotechnology, Rural 
      Development Administration, Suwon 441-707, Korea.
FAU - de Jong, Hans
AU  - de Jong H
FAU - Yang, Tae-Jin
AU  - Yang TJ
FAU - Park, Jee-Young
AU  - Park JY
FAU - Kwon, Soo-Jin
AU  - Kwon SJ
FAU - Kim, Jung Sun
AU  - Kim JS
FAU - Lim, Myung-Ho
AU  - Lim MH
FAU - Kim, Jin A
AU  - Kim JA
FAU - Jin, Mina
AU  - Jin M
FAU - Jin, Yong-Moon
AU  - Jin YM
FAU - Kim, Seog Hyung
AU  - Kim SH
FAU - Lim, Yong Pyo
AU  - Lim YP
FAU - Bang, Jae-Wook
AU  - Bang JW
FAU - Kim, Ho-Il
AU  - Kim HI
FAU - Park, Beom-Seok
AU  - Park BS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Arabidopsis/genetics
MH  - Base Sequence
MH  - Brassica rapa/*genetics
MH  - Chromosomes, Plant/ultrastructure
MH  - DNA, Ribosomal/*analysis
MH  - Heterochromatin/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - *Tandem Repeat Sequences
EDAT- 2005/07/05 09:00
MHDA- 2005/10/28 09:00
CRDT- 2005/07/05 09:00
PHST- 2005/07/05 09:00 [pubmed]
PHST- 2005/10/28 09:00 [medline]
PHST- 2005/07/05 09:00 [entrez]
AID - S1016-8478(23)13190-6 [pii]
PST - ppublish
SO  - Mol Cells. 2005 Jun 30;19(3):436-44.