PMID- 15998435
OWN - NLM
STAT- MEDLINE
DCOM- 20051101
LR  - 20151119
IS  - 1478-3223 (Print)
IS  - 1478-3223 (Linking)
VI  - 25
IP  - 4
DP  - 2005 Aug
TI  - Coordinated expression of cyclin D1 and LEF-1/TCF transcription factor is 
      restricted to a subset of hepatocellular carcinoma.
PG  - 839-47
AB  - BACKGROUND: While the Wnt pathway has been widely implicated in 
      hepatocarcinogenesis, the role of cyclin D1 as a direct downstream target gene of 
      beta-catenin-lymphoid enhancer factor-1 (LEF-1)/T-cell factor (TCF) signaling is 
      controversely discussed. METHODS: By immunohistochemical analyses we studied the 
      subcellular localization of LEF-1/TCF and cyclin D1 in 162 hepatocellular 
      carcinoma (HCC). Single- and double-label imaging by brightfield and confocal 
      laser scanning microscopy was quantitated and correlated with beta-catenin, the 
      Ki67(+) proliferation fraction (PF), tumor size, grade, the Okuda stage and 
      patient survival. RESULTS: The frequency of nuclear cyclin D1 expression was 28% 
      and closely correlated with LEF-1/TCF (P<0.0001) and the Ki67(+) PF (P=0.03). 
      Nuclear LEF-1/TCF expression was observed in 52% of all cases, but was also 
      present in 42% of cyclin D1(-) cases. Nuclear beta-catenin was identified in 37% 
      of all HCCs and correlated with LEF-1/TCF (P=0.04). The expression of cyclin D1, 
      LEF-1/TCF or beta-catenin did not correlate with other clinico-pathological data. 
      CONCLUSIONS: A large proportion of HCCs does not appear to be linked to a 
      deregulation of cyclin D1. However, the coordinated expression of cyclin D1 and 
      LEF-1/TCF in some cases suggests the role of cyclin D1 as a Wnt target gene in a 
      subset of HCCs.
FAU - Schmitt-Graeff, Annette
AU  - Schmitt-Graeff A
AD  - Institute of Pathology, University of Freiburg, Freiburg, Germany. 
      ahsg@ukl.uni-freiburg.de
FAU - Ertelt-Heitzmann, Viktoria
AU  - Ertelt-Heitzmann V
FAU - Allgaier, Hans-Peter
AU  - Allgaier HP
FAU - Olschewski, Manfred
AU  - Olschewski M
FAU - Nitschke, Roland
AU  - Nitschke R
FAU - Haxelmans, Sabine
AU  - Haxelmans S
FAU - Koelble, Konrad
AU  - Koelble K
FAU - Behrens, Jurgen
AU  - Behrens J
FAU - Blum, Hubert Erich
AU  - Blum HE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Liver Int
JT  - Liver international : official journal of the International Association for the 
      Study of the Liver
JID - 101160857
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (LEF1 protein, human)
RN  - 0 (Lymphoid Enhancer-Binding Factor 1)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (beta Catenin)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/metabolism
MH  - Carcinoma, Hepatocellular/*metabolism/mortality/pathology
MH  - Cell Nucleus/metabolism/pathology
MH  - Child
MH  - Cyclin D1/*metabolism
MH  - Cytoskeletal Proteins/metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - Ki-67 Antigen/metabolism
MH  - Liver Neoplasms/*metabolism/mortality/pathology
MH  - Lymphoid Enhancer-Binding Factor 1
MH  - Male
MH  - Microscopy, Confocal
MH  - Middle Aged
MH  - Survival Rate
MH  - Trans-Activators/metabolism
MH  - Transcription Factors/*metabolism
MH  - beta Catenin
EDAT- 2005/07/07 09:00
MHDA- 2005/11/03 09:00
CRDT- 2005/07/07 09:00
PHST- 2005/07/07 09:00 [pubmed]
PHST- 2005/11/03 09:00 [medline]
PHST- 2005/07/07 09:00 [entrez]
AID - LIV1069 [pii]
AID - 10.1111/j.1478-3231.2005.01069.x [doi]
PST - ppublish
SO  - Liver Int. 2005 Aug;25(4):839-47. doi: 10.1111/j.1478-3231.2005.01069.x.