PMID- 1599883
OWN - NLM
STAT- MEDLINE
DCOM- 19920710
LR  - 20011102
IS  - 0921-3775 (Print)
IS  - 0921-3775 (Linking)
VI  - 4
IP  - 1
DP  - 1992
TI  - New optical method for determination of antigen-antibody reaction using 
      level-crossing technique.
PG  - 47-54
AB  - Presented here is a new method of optically detecting agglutination reactions 
      through counting the number of level-crossings of light intensity fluctuations 
      scattered by coated carrier particles. The theory behind this technique is that 
      the number of level-crossings decreases as the size of aggregated particles and, 
      accordingly, the antigen concentration increases. The antigen used in this 
      experiment is immunoglobulin E (IgE). The crossing level is fixed at the average 
      intensity of the scattered light. The detectable range is from 17 to 1000 IU/ml 
      under this condition. The incubation time is 15 min. The coefficient of 
      variations (CVs) of the number of level-crossings is reasonably low, between 
      0.382 and 3.05% (n = 5). Through simple data processing involving comparison of 
      the data with the crossing level and counting the number of level-crossings, it 
      is possible to determine the antigen concentration on a real time basis.
FAU - Namba, A
AU  - Namba A
AD  - Biomedical Research Center, Olympus Optical Co. Ltd, Tokyo, Japan.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Front Med Biol Eng
JT  - Frontiers of medical and biological engineering : the international journal of 
      the Japan Society of Medical Electronics and Biological Engineering
JID - 9011464
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Agglutination Tests/*methods
MH  - *Antigen-Antibody Reactions
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/*analysis
MH  - Mathematics
EDAT- 1992/01/01 00:00
MHDA- 1992/01/01 00:01
CRDT- 1992/01/01 00:00
PHST- 1992/01/01 00:00 [pubmed]
PHST- 1992/01/01 00:01 [medline]
PHST- 1992/01/01 00:00 [entrez]
PST - ppublish
SO  - Front Med Biol Eng. 1992;4(1):47-54.