PMID- 15998971
OWN - NLM
STAT- MEDLINE
DCOM- 20050809
LR  - 20071115
IS  - 1543-1894 (Print)
IS  - 1543-1894 (Linking)
VI  - 115
DP  - 2005
TI  - Sequential fluorescence in situ hybridization analysis for trisomy 12 in B-cell 
      chronic lymphocytic leukemia.
PG  - 231-40
AB  - Tumor-specific chromosomal abnormalities are attracting a large interest owing to 
      the diagnostic, prognostic, and therapeutic importance. The development of 
      molecular techniques, e.g., fluorescence in situ hybridization (FISH), have 
      improved the detection of specific chromosomal abnormalities in chronic 
      lymphocytic leukemic (CLL). By using FISH, the problem with tumor cells with low 
      mitotic rate is avoided since this method readily detects clonal aberrations also 
      in nondividing, interphase cells. Three different types of probes are used: (1) 
      centromeric probes for numerical chromosome abnormalities, (2) whole chromosome 
      paints, and (3) locus-specific probes. The DNA probes are labeled with 
      fluorochromes and the signals yielded are strong enough to enable analysis of 
      interphase cells. These DNA probes may be directed towards any defined 
      chromosomal region and this chapter will in detail describe the FISH method as a 
      detector of trisomy 12 in CLL.
FAU - Hjalmar, Viktoria
AU  - Hjalmar V
AD  - Karolinska Hospital, Stockholm, Sweden.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Med
JT  - Methods in molecular medicine
JID - 101123138
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - Chromosomes, Human, Pair 12/*genetics
MH  - DNA Probes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis/*genetics
MH  - *Trisomy
EDAT- 2005/07/07 09:00
MHDA- 2005/08/10 09:00
CRDT- 2005/07/07 09:00
PHST- 2005/07/07 09:00 [pubmed]
PHST- 2005/08/10 09:00 [medline]
PHST- 2005/07/07 09:00 [entrez]
AID - 1-59259-936-2:231 [pii]
AID - 10.1385/1-59259-936-2:231 [doi]
PST - ppublish
SO  - Methods Mol Med. 2005;115:231-40. doi: 10.1385/1-59259-936-2:231.