PMID- 15999397
OWN - NLM
STAT- MEDLINE
DCOM- 20060203
LR  - 20141120
IS  - 1099-498X (Print)
IS  - 1099-498X (Linking)
VI  - 7
IP  - 11
DP  - 2005 Nov
TI  - In vivo transduction of HIV-1-derived lentiviral particles engineered for 
      macrolide-adjustable transgene expression.
PG  - 1400-8
AB  - BACKGROUND: The molecular merger of latest-generation transduction technologies 
      with advanced transgene control modalities may foster decisive advances in 
      therapeutic reprogramming of somatic cell phenotypes. METHODS: We have engineered 
      self-inactivating HIV-1-based lentiviral expression vectors for reversible 
      macrolide-adjustable transgene expression. RESULTS: Lentiviral particles 
      engineered for macrolide-responsive human vascular endothelial growth factor 121 
      (VEGF121) expression compared favourably with isogenic streptogramin- and 
      tetracycline-responsive configurations and showed excellent growth-factor 
      fine-tuning following transduction into a variety of mammalian cell lines and 
      different human primary cells. Chicken embryos transduced for 
      macrolide-controlled VEGF121 production exhibited dose-dependent 
      neovascularization and exemplified lentivector-delivered transgene transcription 
      fine-tuning in vivo. CONCLUSIONS: Macrolide-adjustable lentivectors enable robust 
      and precise in vitro and in vivo transgene fine-tuning which may give future gene 
      therapy trials a new impetus.
CI  - Copyright (c) 2005 John Wiley & Sons, Ltd.
FAU - Mitta, Barbara
AU  - Mitta B
AD  - Institute for Chemical and Bio-Engineering, Swiss Federal Institute of 
      Technology, ETH Hoenggerberg, HCI F115, Wolfgang-Pauli-Strasse 10, CH-8093 
      Zurich, Switzerland.
FAU - Weber, Cornelia C
AU  - Weber CC
FAU - Fussenegger, Martin
AU  - Fussenegger M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gene Med
JT  - The journal of gene medicine
JID - 9815764
RN  - 0 (Macrolides)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Chick Embryo
MH  - Chorioallantoic Membrane/blood supply/physiology
MH  - Cricetinae
MH  - *Gene Expression Regulation
MH  - *Genetic Engineering
MH  - Genetic Vectors/genetics/metabolism
MH  - HIV-1/*genetics
MH  - Humans
MH  - In Vitro Techniques
MH  - Lentivirus/genetics/*metabolism
MH  - Macrolides/metabolism
MH  - *Transduction, Genetic
MH  - *Transgenes
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
EDAT- 2005/07/07 09:00
MHDA- 2006/02/04 09:00
CRDT- 2005/07/07 09:00
PHST- 2005/07/07 09:00 [pubmed]
PHST- 2006/02/04 09:00 [medline]
PHST- 2005/07/07 09:00 [entrez]
AID - 10.1002/jgm.798 [doi]
PST - ppublish
SO  - J Gene Med. 2005 Nov;7(11):1400-8. doi: 10.1002/jgm.798.