PMID- 16000706
OWN - NLM
STAT- MEDLINE
DCOM- 20051121
LR  - 20200826
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 151
IP  - Pt 7
DP  - 2005 Jul
TI  - Recovery from long-term stationary phase and stress survival in Escherichia coli 
      require the L-isoaspartyl protein carboxyl methyltransferase at alkaline pH.
PG  - 2151-2158
LID - 10.1099/mic.0.27835-0 [doi]
AB  - The L-isoaspartyl protein carboxyl methyltransferase (pcm) can stimulate repair 
      of isoaspartyl residues arising spontaneously in proteins to normal L-aspartyl 
      residues. PCM is needed in Escherichia coli for maximal long-term survival when 
      exposed to oxidative stress, osmotic stress, repeated heat stress or methanol. 
      The effect of pH on a pcm mutant during long-term stationary phase was examined. 
      PCM was not required for long-term survival of E. coli subjected to pH stress 
      alone; however, PCM-deficient cells showed impaired resistance to paraquat and 
      methanol only at elevated pH. The mutant also showed stress-survival phenotypes 
      in minimal medium buffered to pH 9.0. Accumulation of isoaspartyl residues was 
      accelerated at pH 8.0 or 9.0 in vivo, though PCM-deficient cells did not show 
      higher levels of damage. However, the pcm mutant displayed an extended lag phase 
      in recovering from stationary phase at pH 9.0. Protein repair by PCM thus plays a 
      key role in long-term stress survival only at alkaline pH in E. coli, and it may 
      function primarily to repair damage in cells that are recovering from nutrient 
      limitation and in those cells that are able to divide during long-term stationary 
      phase.
FAU - Hicks, Wade M
AU  - Hicks WM
AD  - Department of Biology, North Central College, Naperville, IL 60540, USA.
FAU - Kotlajich, Matthew V
AU  - Kotlajich MV
AD  - Department of Biology, North Central College, Naperville, IL 60540, USA.
FAU - Visick, Jonathan E
AU  - Visick JE
AD  - Department of Biology, North Central College, Naperville, IL 60540, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (Culture Media)
RN  - 0 (Reactive Oxygen Species)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - EC 2.1.1.- (Protein Methyltransferases)
SB  - IM
MH  - Aspartic Acid/*analogs & derivatives
MH  - Bacteriological Techniques
MH  - Culture Media
MH  - Escherichia coli/*enzymology/physiology
MH  - Hydrogen-Ion Concentration
MH  - Protein Methyltransferases/genetics/*metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Recovery of Function
EDAT- 2005/07/08 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/07/08 09:00
PHST- 2005/07/08 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/07/08 09:00 [entrez]
AID - 10.1099/mic.0.27835-0 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2005 Jul;151(Pt 7):2151-2158. doi: 10.1099/mic.0.27835-0.