PMID- 16000770
OWN - NLM
STAT- MEDLINE
DCOM- 20050913
LR  - 20231105
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 71
IP  - 7
DP  - 2005 Jul
TI  - Diversity of bacteria growing in natural mineral water after bottling.
PG  - 3624-32
AB  - Bacterial growth occurs in noncarbonated natural mineral waters a few days after 
      filling and storage at room temperature, a phenomenon known for more than 40 
      years. Using the full-cycle rRNA approach, we monitored the development of the 
      planktonic bacterial community in a noncarbonated natural mineral water after 
      bottling. Seven 16S rRNA gene libraries, comprising 108 clones in total, were 
      constructed from water samples taken at various days after bottling and from two 
      different bottle sizes. Sequence analyses identified 11 operational taxonomic 
      units (OTUs), all but one affiliated with the betaproteobacterial order 
      Burkholderiales (6 OTUs) or the class Alphaproteobacteria (4 OTUs). Fluorescence 
      in situ hybridization (FISH) was applied in combination with DAPI 
      (4',6'-diamidino-2-phenylindole) staining, viability staining, and microscopic 
      counting to quantitatively monitor changes in bacterial community composition. A 
      growth curve similar to that of a bacterium grown in a batch culture was 
      recorded. In contrast to the current perception that Gammaproteobacteria are the 
      most important bacterial components of natural mineral water in bottles, 
      Betaproteobacteria dominated the growing bacterial community and accounted for 80 
      to 98% of all bacteria detected by FISH in the late-exponential and 
      stationary-growth phases. Using previously published and newly designed 
      genus-specific probes, members of the betaproteobacterial genera Hydrogenophaga, 
      Aquabacterium, and Polaromonas were found to constitute a significant proportion 
      of the bacterial flora (21 to 86% of all bacteria detected by FISH). For the 
      first time, key genera responsible for bacterial growth in a natural mineral 
      water were identified by applying molecular cultivation-independent techniques.
FAU - Loy, Alexander
AU  - Loy A
AD  - Abteilung fur Mikrobielle Okologie, Institut fur Okologie und Naturschutz, 
      Universitat Wien, Althanstr. 14, A-1090 Vienna, Austria. 
      loy@microbial-ecology.net
FAU - Beisker, Wolfgang
AU  - Beisker W
FAU - Meier, Harald
AU  - Meier H
LA  - eng
SI  - GENBANK/AF522997
SI  - GENBANK/AF522998
SI  - GENBANK/AF522999
SI  - GENBANK/AF523000
SI  - GENBANK/AF523001
SI  - GENBANK/AF523002
SI  - GENBANK/AF523003
SI  - GENBANK/AF523004
SI  - GENBANK/AF523005
SI  - GENBANK/AF523006
SI  - GENBANK/AF523007
SI  - GENBANK/AF523008
SI  - GENBANK/AF523009
SI  - GENBANK/AF523010
SI  - GENBANK/AF523011
SI  - GENBANK/AF523012
SI  - GENBANK/AF523013
SI  - GENBANK/AF523014
SI  - GENBANK/AF523015
SI  - GENBANK/AF523016
SI  - GENBANK/AF523017
SI  - GENBANK/AF523018
SI  - GENBANK/AF523019
SI  - GENBANK/AF523020
SI  - GENBANK/AF523021
SI  - GENBANK/AF523022
SI  - GENBANK/AF523023
SI  - GENBANK/AF523024
SI  - GENBANK/AF523025
SI  - GENBANK/AF523026
SI  - GENBANK/AF523027
SI  - GENBANK/AF523028
SI  - GENBANK/AF523029
SI  - GENBANK/AF523030
SI  - GENBANK/AF523031
SI  - GENBANK/AF523032
SI  - GENBANK/AF523033
SI  - GENBANK/AF523034
SI  - GENBANK/AF523035
SI  - GENBANK/AF523036
SI  - GENBANK/AF523037
SI  - GENBANK/AF523038
SI  - GENBANK/AF523039
SI  - GENBANK/AF523040
SI  - GENBANK/AF523041
SI  - GENBANK/AF523042
SI  - GENBANK/AF523043
SI  - GENBANK/AF523044
SI  - GENBANK/AF523045
SI  - GENBANK/AF523046
SI  - GENBANK/AF523047
SI  - GENBANK/AF523048
SI  - GENBANK/AF523049
SI  - GENBANK/AF523050
SI  - GENBANK/AF523051
SI  - GENBANK/AF523052
SI  - GENBANK/AF523053
SI  - GENBANK/AF523054
SI  - GENBANK/AF523055
SI  - GENBANK/AF523056
SI  - GENBANK/AF523057
SI  - GENBANK/AF523058
SI  - GENBANK/AF523059
SI  - GENBANK/AF523060
SI  - GENBANK/AF523061
SI  - GENBANK/AF523062
SI  - GENBANK/AF523063
SI  - GENBANK/AF523064
SI  - GENBANK/AF523065
SI  - GENBANK/AF523066
SI  - GENBANK/AF523067
SI  - GENBANK/AF523068
SI  - GENBANK/AF523069
SI  - GENBANK/AF523070
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Indoles)
RN  - 0 (Mineral Waters)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Bacteria/*classification/genetics/*growth & development
MH  - Betaproteobacteria/classification/genetics/growth & development
MH  - Colony Count, Microbial
MH  - Ecosystem
MH  - Food Packaging/methods
MH  - Gene Library
MH  - Genes, rRNA
MH  - *Genetic Variation
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles/metabolism
MH  - Mineral Waters/*microbiology
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Staining and Labeling
PMC - PMC1169067
EDAT- 2005/07/08 09:00
MHDA- 2005/09/15 09:00
PMCR- 2005/07/01
CRDT- 2005/07/08 09:00
PHST- 2005/07/08 09:00 [pubmed]
PHST- 2005/09/15 09:00 [medline]
PHST- 2005/07/08 09:00 [entrez]
PHST- 2005/07/01 00:00 [pmc-release]
AID - 71/7/3624 [pii]
AID - 2635-04 [pii]
AID - 10.1128/AEM.71.7.3624-3632.2005 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2005 Jul;71(7):3624-32. doi: 
      10.1128/AEM.71.7.3624-3632.2005.