PMID- 16002150
OWN - NLM
STAT- MEDLINE
DCOM- 20060120
LR  - 20181201
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 101
IP  - 2
DP  - 2005 Nov 15
TI  - Activation of purified allogeneic CD4(+) T cells by rat bone marrow-derived 
      dendritic cells induces concurrent secretion of IFN-gamma, IL-4, and IL-10.
PG  - 193-201
AB  - Dendritic cells (DCs) are highly specialized antigen-presenting cells that play a 
      key role in the initiation and regulation of immune responses. The ability of DCs 
      to process antigens and the outcome of their interaction with T cells are largely 
      dependent on phenotype as well as maturation state of DCs. In this study, we 
      generated DCs from rat bone marrow precursors. Bone marrow cells cultured in the 
      presence of granulocyte macrophage colony-stimulating factor (GM-CSF), 
      interleukin (IL)-4, and Flt-3 ligand (FL) produced immature DCs that expressed 
      intermediate levels of major histocompatibility complex (MHC) class II, low 
      levels of CD80 and CD86 molecules and displayed a high capacity of endocytosis. 
      Bone marrow-derived DCs (BMDCs) matured in the presence of lipopolysaccharide 
      (LPS) upregulated expression of MHC class II, CD80 and CD86, while their 
      phagocytic capacity was dramatically reduced. Mature BMDCs stimulated vigorous 
      proliferation of purified allogeneic CD4(+) T cells in a primary mixed leukocyte 
      reaction (MLR) and elicited a mixed cytokine profile in allogeneic CD4(+) T 
      cells: DCs activated CD4(+) T cells to produce interferon (IFN)-gamma, IL-4, and 
      IL-10. Thus, rat BMDCs effectively internalize antigens and stimulate T cell 
      proliferation but fail to induce an unidirectional polarization of T helper 
      (T(H)) cells and in this respect differ from both human and mouse DCs.
FAU - Janelidze, Shorena
AU  - Janelidze S
AD  - Glioma Immunotherapy Group, The Rausing Laboratory, Division of Neurosurgery, 
      Department of Clinical Neuroscience, University of Lund, BMC I12, 221 84 Lund, 
      Sweden. Shorena.Janelidze@neurokir.lu.se
FAU - Enell, Karin
AU  - Enell K
FAU - Visse, Edward
AU  - Visse E
FAU - Darabi, Anna
AU  - Darabi A
FAU - Salford, Leif G
AU  - Salford LG
FAU - Siesjo, Peter
AU  - Siesjo P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - CD4-Positive T-Lymphocytes/cytology/*immunology/*metabolism
MH  - Cell Polarity
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Dendritic Cells/*physiology
MH  - Down-Regulation
MH  - Interferon-gamma/*metabolism
MH  - Interleukin-10/*metabolism
MH  - Interleukin-4/*metabolism
MH  - Male
MH  - Phenotype
MH  - Rats
MH  - Th1 Cells/cytology
MH  - Th2 Cells/cytology
EDAT- 2005/07/09 09:00
MHDA- 2006/01/21 09:00
CRDT- 2005/07/09 09:00
PHST- 2004/09/30 00:00 [received]
PHST- 2005/03/24 00:00 [revised]
PHST- 2005/05/31 00:00 [accepted]
PHST- 2005/07/09 09:00 [pubmed]
PHST- 2006/01/21 09:00 [medline]
PHST- 2005/07/09 09:00 [entrez]
AID - S0165-2478(05)00137-9 [pii]
AID - 10.1016/j.imlet.2005.05.012 [doi]
PST - ppublish
SO  - Immunol Lett. 2005 Nov 15;101(2):193-201. doi: 10.1016/j.imlet.2005.05.012.