PMID- 16005165
OWN - NLM
STAT- MEDLINE
DCOM- 20051007
LR  - 20181113
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 356
DP  - 2005 Aug 15
TI  - Asymmetry of intronic pre-miRNA structures in functional RISC assembly.
PG  - 32-8
AB  - The two oligonucleotide strands of a siRNA duplex are functionally asymmetric in 
      assembling the RNAi effector, RNA-induced gene silencing complex (RISC). Based on 
      this asymmetric RISC assembly model in vitro, formation of a microRNA (miRNA) and 
      complementary miRNA (miRNA*) duplex was proposed to be an essential step for the 
      assembly of miRNA-associated RISC (miRISC). We observed here that a strong 
      structural bias exists in the selection of a mature miRNA strand for RISC 
      assembly in zebrafish using an intronic miRNA-like vector to target EGFP mRNA for 
      regulation. The position of the stemloop in a precursor miRNA (pre-miRNA) was 
      involved in the determination of miRNA-miRNA* asymmetry of the pre-miRNA stemarm, 
      leading to different miRNA maturation during miRISC assembly. These findings 
      suggest that the miRISC assembly is likely different from the RISC assembly model 
      of siRNA in zebrafish, providing the first in vivo evidence for asymmetric miRISC 
      assembly.
FAU - Lin, Shi-Lung
AU  - Lin SL
AD  - Department of Cell and Neurobiology, Keck School of Medicine, University of 
      Southern California, 1333 San Pablo Street, BMT-403, Los Angeles, CA 90033, USA. 
      lins@usc.edu
FAU - Chang, Donald
AU  - Chang D
FAU - Ying, Shao-Yao
AU  - Ying SY
LA  - eng
GR  - P40 RR012546/RR/NCRR NIH HHS/United States
GR  - RR12546/RR/NCRR NIH HHS/United States
GR  - CA-85722/CA/NCI NIH HHS/United States
GR  - R01 CA085722-04/CA/NCI NIH HHS/United States
GR  - R01 CA085722/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (MicroRNAs)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Induced Silencing Complex)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Genetic Vectors/genetics
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Introns/*genetics
MH  - MicroRNAs/chemistry/*genetics/metabolism
MH  - Nucleic Acid Conformation
MH  - RNA Interference
MH  - RNA Precursors/chemistry/*genetics
MH  - RNA Splicing
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA-Induced Silencing Complex/chemistry/*genetics
MH  - Transfection
MH  - Zebrafish
PMC - PMC1788082
MID - NIHMS12292
EDAT- 2005/07/12 09:00
MHDA- 2005/10/08 09:00
PMCR- 2008/01/31
CRDT- 2005/07/12 09:00
PHST- 2005/02/15 00:00 [received]
PHST- 2005/04/04 00:00 [revised]
PHST- 2005/04/27 00:00 [accepted]
PHST- 2005/07/12 09:00 [pubmed]
PHST- 2005/10/08 09:00 [medline]
PHST- 2005/07/12 09:00 [entrez]
PHST- 2008/01/31 00:00 [pmc-release]
AID - S0378-1119(05)00251-9 [pii]
AID - 10.1016/j.gene.2005.04.036 [doi]
PST - ppublish
SO  - Gene. 2005 Aug 15;356:32-8. doi: 10.1016/j.gene.2005.04.036.