PMID- 16006146
OWN - NLM
STAT- MEDLINE
DCOM- 20060901
LR  - 20091119
IS  - 1050-4648 (Print)
IS  - 1050-4648 (Linking)
VI  - 20
IP  - 3
DP  - 2006 Mar
TI  - The expression of immune-regulatory genes in rainbow trout, Oncorhynchus mykiss, 
      during amoebic gill disease (AGD).
PG  - 346-64
AB  - Amoebic gill disease (AGD) is an ectoparasitic disease caused by infection with 
      the protozoan Neoparamoeba sp. and is characterised by epithelial hyperplasia 
      that manifests as gill lesions. In order to examine the nature of the immune 
      response to AGD, the expression of a range of immune-regulatory genes was 
      examined in naive uninfected rainbow trout, Oncorhynchus mykiss, and naive 
      rainbow trout subjected to a laboratory-induced AGD infection. The 
      immune-regulatory genes examined were interleukin-1 beta isoform 1 (IL-1beta1), 
      tumour necrosis factor alpha isoforms 1 and 2 (TNF-alpha1, TNF-alpha2), 
      interleukin-8 (IL-8), transforming growth factor beta isoform 1 (TGF-beta1), 
      inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), major 
      histocompatibility complex beta chain (MHC-II beta-chain) and T-cell receptor 
      beta chain (TCR beta-chain). Immune-regulatory genes that were up/down-regulated 
      in AGD-infected trout compared to uninfected controls at 0, 7, and 14 days 
      post-inoculation (p.i.) in gill, liver and anterior kidney tissue were initially 
      identified by means of semi-quantitative RT-PCR. Up/down-regulated 
      immune-regulatory genes were subsequently quantitated and validated by real-time 
      RT-PCR (qRT-PCR). The extent of AGD-associated pathology was consistent amongst 
      all AGD-infected trout at 7 days p.i. and increased considerably by 14 days p.i. 
      At both 7 and 14 days p.i. IL-1beta1 and iNOS gene expression was significantly 
      up-regulated in the gills, and IL-8 was significantly up-regulated in the liver 
      of AGD-infected trout at 7 days p.i. These data demonstrate the involvement of 
      the immune response to AGD at the molecular level, and indicate the importance of 
      this response at the site of infection and the possible involvement of a systemic 
      immune response.
FAU - Bridle, Andrew R
AU  - Bridle AR
AD  - School of Aquaculture, Tasmanian Aquaculture and Fisheries Institute and Aquafin 
      CRC, University of Tasmania, Locked Bag 1370, Launceston 7250, Tasmania, 
      Australia. andrew.bridle@utas.edu.au
FAU - Morrison, Richard N
AU  - Morrison RN
FAU - Nowak, Barbara F
AU  - Nowak BF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - DNA Primers
MH  - Fish Diseases/genetics/*immunology/metabolism/*parasitology
MH  - Gene Expression Regulation/*immunology
MH  - Gills/metabolism/parasitology/pathology
MH  - Interleukin-1/metabolism
MH  - Interleukin-8/metabolism
MH  - *Lobosea
MH  - *Oncorhynchus mykiss
MH  - Protozoan Infections/immunology/metabolism
MH  - *Protozoan Infections, Animal
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Transforming Growth Factor beta/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2005/07/12 09:00
MHDA- 2006/09/02 09:00
CRDT- 2005/07/12 09:00
PHST- 2005/07/12 09:00 [pubmed]
PHST- 2006/09/02 09:00 [medline]
PHST- 2005/07/12 09:00 [entrez]
AID - S1050-4648(05)00097-5 [pii]
AID - 10.1016/j.fsi.2005.05.014 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2006 Mar;20(3):346-64. doi: 10.1016/j.fsi.2005.05.014.