PMID- 16006802
OWN - NLM
STAT- MEDLINE
DCOM- 20050920
LR  - 20220408
IS  - 0147-5185 (Print)
IS  - 0147-5185 (Linking)
VI  - 29
IP  - 8
DP  - 2005 Aug
TI  - Diffuse large B-cell lymphoma with extra Bcl-2 gene signals detected by FISH 
      analysis is associated with a "non-germinal center phenotype".
PG  - 1067-73
AB  - Amplification and translocation of the Bcl-2 gene has been detected in a certain 
      subset of diffuse large B-cell lymphomas (DLBCL). The correlations among Bcl-2 
      protein expression, gene translocation or amplification, and the molecular 
      signature determined by cDNA array are poorly understood. This study examined 25 
      cases with de novo nodal DLBCL. Interphase fluorescence in situ hybridization 
      (FISH) analysis was performed to evaluate the Bcl-2 gene using IGH/BCL2 and CEP18 
      centromere probes (Vysis). When extra Bcl-2 gene signals were observed in each 
      tumor cell and when these signals were in proportion to the extra CEP18 probe 
      signals, we regarded the findings as indicating the presence of an additional 
      chromosome 18; when extra Bcl-2 signals were observed but additional CEP18 
      signals were not, we regarded the findings as indicating the presence of gene 
      amplification. A panel of 3 antigens (CD10, Bcl-6, and MUM-1) was applied to 
      categorize each case as either a "germinal center B-cell (GCB) phenotype" or a 
      "non-GCB phenotype." Of the 25 cases examined, 8 cases (32%) were classified as 
      "GCB phenotype" and 17 cases (68%) were classified as "non-GCB phenotype." A FISH 
      analysis revealed that t(14;18) was detected in 2 of the 8 cases (25%) with the 
      "GCB phenotype" but in none of the 17 "non-GCB phenotype" cases. Extra Bcl-2 gene 
      signals were detected in 7 of the 25 (28%) cases examined: n = 5 for an 
      additional chromosome 18, n = 1 for gene amplification, and n = 1 for additional 
      chromosome 18 + gene amplification. Extra Bcl-2 gene signals were exclusively 
      detected in DLBCL with the "non-GCB phenotype"; these cases, with the exception 
      of one, stained strongly positive for Bcl-2. The DLBCLs with Bcl-2 protein 
      overexpression were classified into at least two heterogeneous molecular groups, 
      based on the results of the FISH analysis.
FAU - Kusumoto, Shigeru
AU  - Kusumoto S
AD  - Hematology Division, National Cancer Center Hospital, Chuo-ku, Tokyo, Japan.
FAU - Kobayashi, Yukio
AU  - Kobayashi Y
FAU - Sekiguchi, Naohiro
AU  - Sekiguchi N
FAU - Tanimoto, Kazuki
AU  - Tanimoto K
FAU - Onishi, Yasushi
AU  - Onishi Y
FAU - Yokota, Yukiko
AU  - Yokota Y
FAU - Watanabe, Takashi
AU  - Watanabe T
FAU - Maeshima, Akiko Miyagi
AU  - Maeshima AM
FAU - Ishida, Takashi
AU  - Ishida T
FAU - Inagaki, Hiroshi
AU  - Inagaki H
FAU - Matsuno, Yoshihiro
AU  - Matsuno Y
FAU - Ueda, Ryuzo
AU  - Ueda R
FAU - Tobinai, Kensei
AU  - Tobinai K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Line, Tumor
MH  - Chromosomes, Human, Pair 14
MH  - Chromosomes, Human, Pair 18
MH  - Female
MH  - Gene Amplification
MH  - Genes, bcl-2/*physiology
MH  - Germinal Center/physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell/*genetics/therapy
MH  - Lymphoma, Large B-Cell, Diffuse/*genetics/therapy
MH  - Male
MH  - Middle Aged
EDAT- 2005/07/12 09:00
MHDA- 2005/09/21 09:00
CRDT- 2005/07/12 09:00
PHST- 2005/07/12 09:00 [pubmed]
PHST- 2005/09/21 09:00 [medline]
PHST- 2005/07/12 09:00 [entrez]
AID - 00000478-200508000-00011 [pii]
PST - ppublish
SO  - Am J Surg Pathol. 2005 Aug;29(8):1067-73.