PMID- 16006805
OWN - NLM
STAT- MEDLINE
DCOM- 20050920
LR  - 20151119
IS  - 0147-5185 (Print)
IS  - 0147-5185 (Linking)
VI  - 29
IP  - 8
DP  - 2005 Aug
TI  - Clinical, immunophenotypic, and genetic analysis of adult lymphomas with 
      morphologic features of Burkitt lymphoma.
PG  - 1086-94
AB  - A prompt distinction of Burkitt lymphoma (BL) versus diffuse large B cell 
      lymphomas (DLBCL) has important clinical implications; however, this distinction 
      can be difficult. We analyzed 74 adult gray zone and 10 reference pediatric BL 
      using immunohistochemistry (Ki-67, CD10, bcl2, bcl6) and fluorescence in situ 
      hybridization (FISH) for MYC, BCL2, and BCL6 breakpoints. Two algorithms for 
      classification were followed: algorithm A used a two-step review by four 
      hemato-pathologists and algorithm B a set of only biologic markers (Ki-67 > or = 
      90%, CD10+, bcl6+, bcl2-, MYC breakpoint+, BCL2 and BCL6 breakpoint-). Both 
      algorithms categorized all reference cases as BL. In the adult group, algorithm A 
      resulted in 21 adult BL and 52 DLBCL and algorithm B in 23 BL and 51 
      "non-Burkitt" lymphomas (nBL); 9 cases (12%) contained two different 
      translocations and were categorized as nBL in algorithm B. Fifteen cases (20%) 
      fulfilled the BL criteria of both algorithms. Although not considered as BL 
      according to both algorithms, many other lymphomas showed nonetheless a 
      phenotypic and/or genetic shift to BL. BL according to algorithm B was more 
      homogeneous with respect to clinical presentation (gender and localization) than 
      BL defined by algorithm A. Our data suggest that only a few cases of these gray 
      zone lymphomas represent true de novo BL. Immunohistochemistry for Ki-67, CD10, 
      and bcl2 with analysis of MYC and preferably also BCL2 and BCL6 may be advised as 
      a marker panel for this diagnostic dilemma.
FAU - Haralambieva, Eugenia
AU  - Haralambieva E
AD  - Department of Pathology, University Medical Center Groningen, Groningen, The 
      Netherlands.
FAU - Boerma, Evert-Jan
AU  - Boerma EJ
FAU - van Imhoff, Gustaaf W
AU  - van Imhoff GW
FAU - Rosati, Stefano
AU  - Rosati S
FAU - Schuuring, Ed
AU  - Schuuring E
FAU - Muller-Hermelink, Hans Konrad
AU  - Muller-Hermelink HK
FAU - Kluin, Philip M
AU  - Kluin PM
FAU - Ott, German
AU  - Ott G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Proto-Oncogene Proteins c-bcl-6)
RN  - 0 (Transcription Factors)
RN  - EC 3.4.24.11 (Neprilysin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Algorithms
MH  - Biomarkers, Tumor/analysis
MH  - Burkitt Lymphoma/diagnosis/*genetics/pathology
MH  - Child
MH  - Child, Preschool
MH  - DNA-Binding Proteins/analysis
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - Ki-67 Antigen/analysis
MH  - Lymphoma/*genetics/*immunology/pathology
MH  - Male
MH  - Middle Aged
MH  - Neprilysin/analysis
MH  - Proto-Oncogene Proteins/analysis
MH  - Proto-Oncogene Proteins c-bcl-2/analysis
MH  - Proto-Oncogene Proteins c-bcl-6
MH  - Transcription Factors/analysis
MH  - Translocation, Genetic
EDAT- 2005/07/12 09:00
MHDA- 2005/09/21 09:00
CRDT- 2005/07/12 09:00
PHST- 2005/07/12 09:00 [pubmed]
PHST- 2005/09/21 09:00 [medline]
PHST- 2005/07/12 09:00 [entrez]
AID - 00000478-200508000-00014 [pii]
PST - ppublish
SO  - Am J Surg Pathol. 2005 Aug;29(8):1086-94.