PMID- 16006966
OWN - NLM
STAT- MEDLINE
DCOM- 20050916
LR  - 20050711
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 174
IP  - 2
DP  - 2005 Aug
TI  - Identification of tumor entities of renal cell carcinoma using interphase 
      fluorescence in situ hybridization.
PG  - 731-5
AB  - PURPOSE: We developed a rapid interphase fluorescence in situ hybridization 
      (FISH) test to differentiate renal cell carcinoma (RCC) based on known genetic 
      alterations and verified the suitability of this test for practical use. 
      MATERIALS AND METHODS: We composed 2 FISH test sets using 6 centromere specific 
      and 2 region specific DNA probes of human chromosomes. Test set 1 contained 
      centromeric probes for chromosomes 1, 2, 6 and 9, as labeled by 4 fluorescence 
      dyes. For test set 2 we chose 3p24pter and 3p13p14 regions, and centromeric 
      probes of chromosomes 7 and 17. Interphase nuclei of tumor specimens were 
      prepared from 50 mum frozen tissue sections and fixed on slides. The 2 sets were 
      hybridized simultaneously side by side on the same slide. RESULTS: Seven clear 
      cell carcinomas, 8 papillary carcinomas, 7 chromophobe RCCs and 3 oncocytomas 
      were analyzed by interphase FISH. Results were compared with comparative genomic 
      hybridization findings and pathological reports. Genetic alterations were 
      detected in 22 of 25 analyzed tumors by FISH. FISH findings absolutely correlated 
      with comparative genomic hybridization results. Of the analyzed carcinomas 22 
      could be identified correctly. In 3 tumors the histological subtypes were 
      revised. CONCLUSIONS: The results of this study demonstrate that the performed 
      test set allows the accurate identification of RCC in 1 hybridization step. 
      Therefore, FISH represents an effective method for the rapid classification of 
      RCC.
FAU - Sanjmyatav, Jimsgene
AU  - Sanjmyatav J
AD  - Department of Urology, Friedrich-Schiller-University, Jena, Germany. 
      jimsgene.sanjmyatav@med.uni-jena.de
FAU - Rubtsov, Nikolai
AU  - Rubtsov N
FAU - Starke, Heike
AU  - Starke H
FAU - Schubert, Joerg
AU  - Schubert J
FAU - Hindermann, Winfried
AU  - Hindermann W
FAU - Junker, Kerstin
AU  - Junker K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
SB  - IM
MH  - Carcinoma, Renal Cell/*diagnosis
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Kidney Neoplasms/*diagnosis
MH  - Sensitivity and Specificity
EDAT- 2005/07/12 09:00
MHDA- 2005/09/17 09:00
CRDT- 2005/07/12 09:00
PHST- 2005/07/12 09:00 [pubmed]
PHST- 2005/09/17 09:00 [medline]
PHST- 2005/07/12 09:00 [entrez]
AID - S0022-5347(01)68381-2 [pii]
AID - 10.1097/01.ju.0000166749.38455.bc [doi]
PST - ppublish
SO  - J Urol. 2005 Aug;174(2):731-5. doi: 10.1097/01.ju.0000166749.38455.bc.